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Rescue of high-specificity Cas9 variants using sgRNAs with matched 5 ' nucleotides
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sojung | - |
dc.contributor.author | Bae, Taegeun | - |
dc.contributor.author | Hwang, Jaewoong | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2017-11-23T07:33:47Z | - |
dc.date.available | 2017-11-23T16:34:52Z | - |
dc.date.created | 2018-07-17 | - |
dc.date.created | 2018-07-17 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.citation | Genome Biology, Vol.18, p. 218 | - |
dc.identifier.issn | 1474-760X | - |
dc.identifier.other | 40534 | - |
dc.identifier.uri | https://hdl.handle.net/10371/138400 | - |
dc.description.abstract | We report that engineered Cas9 variants with improved specificity-eCas9-1.1 and Cas9-HF1-are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5' terminus, relative to target DNA sequences. Because the nucleotide at the 5' end of sgRNAs, expressed under the control of the commonly-used U6 promoter, is fixed to a guanine, these attenuated Cas9 variants are not useful at many target sites. By using sgRNAs with matched 5' nucleotides, produced by linking them to a self-cleaving ribozyme, the editing activity of Cas9 variants can be rescued without sacrificing high specificity. | - |
dc.language | 영어 | - |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central Ltd. | - |
dc.title | Rescue of high-specificity Cas9 variants using sgRNAs with matched 5 ' nucleotides | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1186/s13059-017-1355-3 | - |
dc.citation.journaltitle | Genome Biology | - |
dc.identifier.wosid | 000415306700002 | - |
dc.identifier.scopusid | 2-s2.0-85034565473 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s). | - |
dc.date.updated | 2017-11-19T04:28:46Z | - |
dc.citation.startpage | 218 | - |
dc.citation.volume | 18 | - |
dc.identifier.sci | 000415306700002 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | GUIDE RNAS | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | NUCLEASES | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordAuthor | CRISPR-Cas | - |
dc.subject.keywordAuthor | Off-target effect | - |
dc.subject.keywordAuthor | Engineered Cas9 variants | - |
dc.subject.keywordAuthor | Hammerhead ribozyme-linked sgRNA | - |
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