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Enhanced osteogenic commitment of murine mesenchymal stem cells on graphene oxide substrate

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Authors
Kim, Jiyong; Kim, Hwan D.; Park, Jungha; Lee, Eun-seo; Kim, Eugene; Lee, Seunghun S.; Yang, Jin-Kyung; Lee, Yoon-Sik; Hwang, Nathaniel S.
Issue Date
2018-01-02
Publisher
BioMed Central
Citation
Biomaterials Research, 22(1):1
Keywords
GrapheneOsteogenesisMesenchymal stem cellsGraphene oxide
Abstract
Background
Tissue engineering is an interdisciplinary field that attempts to restore or regenerate tissues and organs through biomimetic fabrication of scaffolds with specific functionality. In recent years, graphene oxide (GO) is considered as promising biomaterial due to its nontoxicity, high dispersity, and hydrophilic interaction, and these characteristics are key to stimulating the interactions between substrates and cells.

Method
In this study, GO substrates were fabricated via chemically immobilizing GO at 1.0 mg/ml on glass slides. Furthermore, we examined the osteogenic responses of murine mesenchymal-like stem cells, C3H10T1/2 cells, on GO substrates.

Results
C3H10T1/2 cells on GO substrates resulted in increased cell surface area, enhanced cellular adhesions, and instigated osteogenic differentiation. Furthermore, priming of C3H10T1/2 cells with chondrocyte-conditioned medium (CM) could further induce a synergistic effect of osteogenesis on GO substrates.

Conclusions
All of these data suggest that GO substrate along with CM is suitable for upregulating osteogenic responses of mesenchymal stem cells.
ISSN
2055-7124
Language
English
URI
http://hdl.handle.net/10371/138483
DOI
https://doi.org/10.1186/s40824-017-0112-8
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College of Engineering/Engineering Practice School (공과대학/대학원)Program in Bioengineering (협동과정-바이오엔지니어링전공)Journal Papers (저널논문_협동과정-바이오엔지니어링전공)
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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