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Nucleic Acid Extraction Method Using SiNx Nanofilter Device : Nucleic Acid Extraction Method Using SiNx Nanofilter Device
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김기범 | - |
dc.contributor.author | 이다은 | - |
dc.date.accessioned | 2018-05-29T03:25:22Z | - |
dc.date.available | 2018-05-29T03:25:22Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.other | 000000150444 | - |
dc.identifier.uri | https://hdl.handle.net/10371/141482 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 공과대학 재료공학부, 2018. 2. 김기범. | - |
dc.description.abstract | Current diagnosis of diseases is an important issue in the biological field. The diagnosis of the disease consists of four stages. Sampling, genetic material separation, amplification and analysis for genetic material. In this study, we tried to isolate the genetic material with a new idea. Since DNA has negative charge, it would move in the electric field. In this basic principle, in one direction electric field, genetic material would be collected in a chamber. However, in the electric field, all charged materials would move but because of high charge-to-mass ratio in DNA, it would transfer fast and firstly. The advantage of this system is that it is a fast, one-step process and minimizes damage to the DNA while minimizing the use of chemical solutions.
In this study, we developed a filter membrane to embody this idea. This filter membrane has millions of pores of 50-200 nm in size, which do not interfere with DNA migration and interfere with other cell debris and proteins. This study was carried out from the chamber design because there was no preceding study. The design of the chamber was confirmed by simulating the pattern of the electric field. Later, to better understand the trends according to the experimental variables, the experimental results were predicted and compared using the model. However, there was a difference between the experimental data and the numerical modeling values because of limits of the numerical model. | - |
dc.description.tableofcontents | Chapter 1. Introduction 1
1-1 Introduction of DNA extraction 2 1-2 Concept of nanofilter system 6 1-3 Outline of the dissertation 8 1-4 References 9 Chapter 2. Nanofilter Fabrication 10 2-1 Fabrication process 11 2-2 Fabrication results 14 Chapter 3. Chamber Design 18 3-1 Introduction 19 3-2 COMSOL Multiphysics Simulation 19 3-3 Results 24 Chapter 4. Numerical Modeling of DNA Translocation and Experimental Results 27 4-1 Introduction 28 4-2 Numerical modeling 28 4-3 Experimental results 32 4-4 Discussion 45 Chapter 5. Summary and Conclusion 47 Abstract (In Korean) 49 | - |
dc.format | application/pdf | - |
dc.format.extent | 2113219 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Nanofilter | - |
dc.subject | DNA extraction | - |
dc.subject | Electric field | - |
dc.subject | Chamber design | - |
dc.subject.ddc | 620.1 | - |
dc.title | Nucleic Acid Extraction Method Using SiNx Nanofilter Device | - |
dc.title.alternative | Nucleic Acid Extraction Method Using SiNx Nanofilter Device | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Da Eun Lee | - |
dc.description.degree | Master | - |
dc.contributor.affiliation | 공과대학 재료공학부 | - |
dc.date.awarded | 2018-02 | - |
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