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Identification of genes inducing resistance to ionizing radiation in human rectal cancer cell lines: re-sensitization of radio-resistant rectal cancer cells through down regulating NDRG1

Cited 5 time in Web of Science Cited 7 time in Scopus
Authors
Kim, Soon-Chan; Shin, Young-Kyoung; Kim, Ye-Ah; Jang, Sang-Geun; Ku, Ja-Lok
Issue Date
2018-05-25
Publisher
BioMed Central
Citation
BMC Cancer, 18(1):594
Keywords
Rectal cancerPaired rectal cancer cell linesEstablishmentRadiationResistanceGene expressionNDRG1ERRFI1Microarray
Abstract
Background
Resistance to preoperative radiotherapy is a major clinical problem in the treatment for locally advanced rectal cancer. The role of NDRG1 in resistance to ionizing radiation in rectal cancer has not been fully elucidated. This study aimed to investigate the effect of the reduced intracellular NDRG1 expression on radio-sensitivity of human rectal cancer cells for exploring novel approaches for treatment of rectal cancer.

Methods
Three radio-resistant human rectal cancer cell lines (SNU-61R80Gy, SNU-283R80Gy, and SNU-503R80Gy) were established from human rectal cancer cell lines (SNU-61, SNU-283, and SNU-503) using total 80 Gy of fractionated irradiation. Microarray analysis was performed to identify differently expressed genes in newly established radio-resistant human rectal cancer cells compared to parental rectal cancer cells.

Results
A microarray analysis indicated the RNA expression of five genes (NDRG1, ERRFI1, H19, MPZL3, and UCA1) was highly increased in radio-resistant rectal cancer cell lines. Short hairpin RNA-mediated silencing of NDRG1 sensitized rectal cancer cell lines to clinically relevant doses of radiation by causing more DNA double strand breakages to rectal cancer cells when exposed to radiation.

Conclusions
Targeting NDRG1 represents a promising strategy to increase response to radiotherapy in human rectal cancer.
ISSN
1471-2407
Language
English
URI
http://hdl.handle.net/10371/142746
DOI
https://doi.org/10.1186/s12885-018-4514-3
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biomedical Sciences (대학원 의과학과)Journal Papers (저널논문_의과학과)
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