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Extension of biotic ligand model to the prediction of site-specific ecological risk of arsenate in Aliivibrio fischeri and Hordeum vulgare : Aliivibrio fischeri 및 Hordeum vulgare에 대한 비소의 현장특이적 생태위해성 예측을 위한 biotic ligand model의 확장
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 남경필 | - |
| dc.contributor.author | 안진성 | - |
| dc.date.accessioned | 2018-11-12T00:56:44Z | - |
| dc.date.available | 2018-11-12T00:56:44Z | - |
| dc.date.issued | 2018-08 | - |
| dc.identifier.other | 000000153494 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/143121 | - |
| dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 공과대학 건설환경공학부, 2018. 8. 남경필. | - |
| dc.description.abstract | The effectiveness of in situ stabilization in arsenic (As)-contaminated soil, as one of risk mitigation measures, should be evaluated by means of both chemical extractability and biological responses, as chemical analysis alone may not be sufficient to assess the ecotoxicity. Although bioassays are liable and realistic in determining As toxicity in soil, they are time-consuming and costly. As an alternative, a biotic ligand model (BLM) was developed to predict the site-specific toxicity of inorganic arsenate (iAs(V)) in soil porewater by using the bioluminescent bacteria Aliivibrio fischeri. To enhance the accuracy of the BLM, the effects of major cations/anions | - |
| dc.description.tableofcontents | Chapter 1 Introduction 1
1.1 General overview 1 1.2 Background 3 1.2.1 Mathematical description of BLM for cationic metals 3 1.2.2 Soil solution toxicity as an indicator of soil toxicity 5 1.2.3 In situ stabilization as a risk mitigation measure for As-contaminated soil 7 1.3 Research objectives 8 References 10 Chapter 2 Evaluation of the Effectiveness of In Situ Stabilization in the Field Aged Arsenic-Contaminated Soil: Chemical Extractability and Biological Responses 14 2.1 Introduction 14 2.2 Materials and methods 16 2.2.1 In situ stabilization 16 2.2.2 Chemical extractability and human health risk characterization 18 2.2.3 Ecotoxicity test with H. vulgare as an indicator of biological responses 21 2.2.4 X-ray absorption spectroscopy 21 2.2.5 Soil physicochemical properties 22 2.3 Results and discussion 23 2.3.1 Chemical extractability and human health risk 23 2.3.2 Biological responses 28 2.4 Summary 32 References 33 Chapter 3 Extension of Biotic Ligand Model to Account for the Effects of pH and Phosphate in Accurate Prediction of Arsenate Toxicity 38 3.1 Introduction 38 3.2 Materials and methods 40 3.2.1 Toxicity test 40 3.2.2 Reagents and sample preparation 41 3.2.3 Effect of pH and iAs(V) toxicity 42 3.2.4 Effect of major cations and anions on iAs(V) toxicity 42 3.2.5 Derivation of iAs(V) toxicity prediction model parameters 43 3.2.6 Chemical analysis 44 3.3 Results and discussion 45 3.3.1 pH dependency of iAs(V) toxicity 45 3.3.2 Effect of cations on iAs(V) toxicity 46 3.3.3 Effect of anions on iAs(V) toxicity 47 3.3.4 Development of extended BLM for prediction of iAs(V) toxicity 49 3.3.5 Validation of extended BLM 55 3.4 Summary 57 References 58 Chapter 4 Use of the Extrapolated Biotic Ligand Model to Predict Arsenate Toxicity in Barley Hordeum vulgare Considering Cell Membrane Surface Electrical Potential 64 4.1 Introduction 64 4.2 Materials and methods 67 4.2.1 Ecotoxicity test with H. vulgare 67 4.2.2 Calculation of the electrical potential of root cell plasma membrane surface 70 4.2.3 Interspecies extrapolation of BLM 72 4.3 Results and discussion 73 4.3.1 Effect of increasing Ca2+ concentrations on iAs(V) toxicity to H. vulgare 73 4.3.2 Interspecies extrapolation of BLM to predict iAs(V) toxicity to H. vulgare 77 4.4 Summary 81 References 82 Chapter 5 A Direct Measurement of Bioavailable Arsenic Concentrations Using Diffusive Gradients in Thin Film and X-ray Fluorescence Spectrometry 86 5.1 Introduction 86 5.2 Materials and methods 89 5.2.1 Diffusive gradient in thin film (DGT) 89 5.2.2 X-ray fluorescence spectrometry (XRF) 90 5.2.3 Calibration and the correction for spectral interference 90 5.2.4 Preparation of freshly spiked and aged soils 91 5.2.5 Phytotoxicity tests with barley H. vulgare 92 5.2.6 Application of the DGT-XRF technique to determine bioavailable As concentrations in soil 93 5.3 Results and discussion 93 5.3.1 Calibration and analytical performance 93 5.3.2 Correction factor for compensating spectral interference of Pb 94 5.3.3 Determination of bioavailable As concentrations in freshly spiked and aged soils 97 5.4 Summary 99 References 100 Chapter 6 Conclusions 105 국문초록 108 | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject.ddc | 624 | - |
| dc.title | Extension of biotic ligand model to the prediction of site-specific ecological risk of arsenate in Aliivibrio fischeri and Hordeum vulgare | - |
| dc.title.alternative | Aliivibrio fischeri 및 Hordeum vulgare에 대한 비소의 현장특이적 생태위해성 예측을 위한 biotic ligand model의 확장 | - |
| dc.type | Thesis | - |
| dc.description.degree | Doctor | - |
| dc.contributor.affiliation | 공과대학 건설환경공학부 | - |
| dc.date.awarded | 2018-08 | - |
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