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Studies on the modulation of autophagic proteolysis during oxidative stress : 산화적 스트레스 상황 하에서 세포 내 오토파지에 의한 단백질 분해 조절에 대한 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이준호 | - |
| dc.contributor.author | 김대호 | - |
| dc.date.accessioned | 2018-11-12T01:00:31Z | - |
| dc.date.available | 2018-11-12T01:00:31Z | - |
| dc.date.issued | 2018-08 | - |
| dc.identifier.other | 000000152058 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/143285 | - |
| dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 자연과학대학 생물물리 및 화학생물학과, 2018. 8. 이준호. | - |
| dc.description.abstract | Reactive oxygen species (ROS) are baring an extra electron, and the electron has a potential to engage in various reactions with its electro-potential energy in the cells. But, when excessive ROS are generated in cells, these ROS react with cellular substances and induce oxidative stress to cells. In response to the stress, there are the oxidative stress scavengers and through these mechanisms, cells could have reduced the concentration of ROS in cells | - |
| dc.description.tableofcontents | ABSTRACT…………………………………………………..i
CONTENTS………...………...……………….…..…....iv LIST OF FIGURES AND TABLES………….…..…....iv ABBREVIATIONS….………………..……….…..…....iv 1. Introduction….……………….……..……….…..…....1 2. Material and methods ….……………….……..…....9 Cell culture………………………………………………………………9 Reagents and antibodies…………………………...………………………9 Production of anti-R-BiP antibody…………………….…………………10 Plasmids, cloning, and mutagenesis..……………………………………10 Stable transfection and generation of stable cell lines………………….…11 DJ-1 complex purification and mass spectrometry analysis……………...12 Subcellular fractionation…………………………………………….…...13 Co-immunoprecipitation…………………………………………………14 Immunostaining assay……………………………………………………14 Detection of ROS generation……………………………………………..15 Immunoblotting assay……………………………………………………15 GST pull-down assay…………………………………………………….16 JC-1 mitochondrial membrane potential assay…………………………...16 Survival assay…………………………………………………………….16 Apoptosis assay…………………………………………………………..17 RT-PCR analysis…………………………………………………………17 Statistical analysis………………………………………………………..18 3. Results…………………….……………….……..…..19 TRAIL induced oxidative stress………………………………………….19 DJ-1 as a TRAIL induced oxidative stress reducer.………………………20 The binding partner of DJ-1 under TRAIL induced oxidative stress...……21 R-BiP induction by TRAIL and interaction with DJ-1…..………………22 Importance of arginylation on BiP in interaction with DJ-1………………23 Oxidation of DJ-1 under the oxidative stress the function of oxidized DJ-1…………......................................………………………………….24 Interaction of oxidized DJ-1 and autophagic receptor p62…......................25 Importance of DJ-1 while the interaction of R-BiP and p62.......…………26 DJ-1, a modulator of R-BiP / p62 autophagic puncta formation .…………27 DJ-1, the function as a modulator of cargo targeting.......…………………28 Cargo delivery defect in DJ-1 deficient cells.....………………………….30 Autophagic proteolysis defect in DJ-1 deficient cells.……………………31 Mitochondria deficiency in DJ-1 deficient cells.…………………………32 Protective role of DJ-1 under ER stress.………………………………….33 Protective role of N-end rule substrate BiP under TRAIL induced stress...35 4. Discussion………………….……………….……….111 5. References………………….……………….……....116 ABSTRACT IN KOREAN / 국문 초록…………….134 | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject.ddc | 571 | - |
| dc.title | Studies on the modulation of autophagic proteolysis during oxidative stress | - |
| dc.title.alternative | 산화적 스트레스 상황 하에서 세포 내 오토파지에 의한 단백질 분해 조절에 대한 연구 | - |
| dc.type | Thesis | - |
| dc.contributor.AlternativeAuthor | Daeho Kim | - |
| dc.description.degree | Doctor | - |
| dc.contributor.affiliation | 자연과학대학 생물물리 및 화학생물학과 | - |
| dc.date.awarded | 2018-08 | - |
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