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Synergistic Anti-Skin Aging Effect of Adipose-derived Stem Cell and Fat Graft : 노화된 생쥐에서 지방줄기세포와 지방이식의 항피부노화 상승효과
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김석화 | - |
| dc.contributor.author | 김기갑 | - |
| dc.date.accessioned | 2018-11-12T01:00:49Z | - |
| dc.date.available | 2018-11-12T01:00:49Z | - |
| dc.date.issued | 2018-08 | - |
| dc.identifier.other | 000000151726 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/143298 | - |
| dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과대학 의학과, 2018. 8. 김석화. | - |
| dc.description.abstract | Background: We investigated the beneficial effects of adipose-derived stem cells (ADSCs) and fat graft on skin wrinkles in a nude mouse model of chronological aging.
Methods: With 50 weeks of chronological aging, 44 female BALB/c-nude mice were classified into 4 groups, i.e., a negative control group and groups injected subcutaneously with ADSCs (1 × 105 cells in 0.5 ml) on the back, injected with fat tissue (0.5 ml), or injected with both fat tissue (0.5 ml) and ADSCs (1 × 105 cells in 0.5ml). The degree of wrinkling was evaluated by a replica analysis and skin biopsies were performed after 4 weeks. The dermal thickness and density of collagen were determined. The levels of type 1 collagen and MMP were determined by western blot and type 1 procollagen mRNA levels were evaluated by RT-PCR. Tropoelastin, fibrillin-1, and CD31 were evaluated by immunohistochemistry. Results: Based on the total wrinkle area, there was significant wrinkle reduction in the fat group and the ADSC with fat group. Collagen expression and type I procollagen mRNA levels were significantly higher in the ADSC with fat group than in the ADSC group and in the fat group. In addition, the ADSC with fat group exhibited significantly higher CD31 expression levels than those in the ADSC group and in the fat group. Conclusions: ADSCs and fat could both play important roles in wrinkle reduction and have synergic effects on collagen synthesis and neovascularization. | - |
| dc.description.tableofcontents | CONTENTS
Abstract ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ і List of figures ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ iii Ⅰ. Introduction ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 1 Ⅱ. Materials and Methods ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 3 Ⅲ. Results ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 9 Ⅳ. Discussion ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 27 Ⅴ. Conclusion ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 32 References ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 33 Abstract in Korean ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ ∙ 40 | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject.ddc | 610 | - |
| dc.title | Synergistic Anti-Skin Aging Effect of Adipose-derived Stem Cell and Fat Graft | - |
| dc.title.alternative | 노화된 생쥐에서 지방줄기세포와 지방이식의 항피부노화 상승효과 | - |
| dc.type | Thesis | - |
| dc.contributor.AlternativeAuthor | Kikap Kim | - |
| dc.description.degree | Doctor | - |
| dc.contributor.affiliation | 의과대학 의학과 | - |
| dc.date.awarded | 2018-08 | - |
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