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Regulation of EXLA1 Stability by E3 Ubiquitin Ligase GW2 in Rice
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 서학수 | - |
| dc.contributor.author | 최범석 | - |
| dc.date.accessioned | 2018-12-03T01:50:42Z | - |
| dc.date.available | 2018-12-03T01:50:42Z | - |
| dc.date.issued | 2018-08 | - |
| dc.identifier.other | 000000151700 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/144077 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 농업생명과학대학 식물생산과학부(작물생명과학전공), 2018. 8. 서학수. | - |
| dc.description.abstract | Grain Weight 2 (GW2), known as the E3 ubiquitin ligase, important role in
controlling the size and weight of the grains. Such gene expression and regulation by GW2 has been studied for a long time. Nevertheless, the post-translational research of GW2 rarely progressed. Previous studies have confirmed in vitro that Grain Weight 2 (GW2) in rice could act as an E3 ubiquitin ligase for EXPANSIN LIKE 1 (EXLA1). Here, we showed that Grain Weight2 (GW2) could regulation EXLA1 stability. In this paper, particle bombardment experiments showed that GW2 and EXLA1 interacted in epidermal cells of onion. The yeast two-hybrid experiment also confirmed that the expansin family except EXLA1 did not bind to GW2. In addition, the expression of EXLA1 was found to be high in the gw2 mutant and the expression of EXLA1 was found to be different when GA3, Cytokinin, and Auxin were treated to the WT Norin. We also found that BSG1, a candidate group other than EXLA1, binds to GW2 through yeast two-hybrid experiments and in vitro pull down experiments. Taken together, we confirmed that the stability of EXLA1 was regulated by GW2, and confirmed that it binds to a candidate group other than EXLA1. | - |
| dc.description.tableofcontents | CONTENTS
ABSTRACT........................................................................................ I CONTENTS .................................................................................... II LIST OF FIGURES ........................................................................ III LIST OF ABBREVIATIONS ......................................................... IV INTRODUCTION ............................................................................. 1 MATERAIALS AND METHODS .................................................... 4 Yeast two-hybrid assay........................................................................... 4 Subcellular localization of GW2 and EXLA1 ............................................. 4 Bacteria and plant mateirals and growth condition ....................................... 5 Construction of recombinent plasmids ...................................................... 5 Purification of recombinent proteins ......................................................... 6 In vitro pull-down assay ......................................................................... 6 Phylogenetic analysis............................................................................. 7 RESULTS ........................................................................................... 8 Yeast two hybrid assay of GW2 and expansin family .................................. 8 Co-Localization of CFP-tagged OsGW2 and YFP–tagged OsEXLA1 in onion epidermal cells ........................................................................... 11 EXLA1 Transcriptional Expression in gw2 mutant and after GA3, Cytokinin, Auxin treatment ......................................................................... 13 Interaction of BSG1 with GW2 ............................................................ 15 DISCUSSSION ................................................................................. 18 REFERENCES ................................................................................ 21 ABSTRACT IN KOREAN .............................................................. 24 | - |
| dc.format | application/pdf | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject.ddc | 633 | - |
| dc.title | Regulation of EXLA1 Stability by E3 Ubiquitin Ligase GW2 in Rice | - |
| dc.type | Thesis | - |
| dc.description.degree | Master | - |
| dc.contributor.affiliation | 농업생명과학대학 식물생산과학부(작물생명과학전공) | - |
| dc.date.awarded | 2018-08 | - |
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