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Comparative transcriptome analysis identified candidate genes involved in mycelium browning in Lentinula edodes

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Authors
Yoo, Seung-il; Lee, Hwa-Yong; Markkandan, Kesavan; Moon, Suyun; Ahn, Yong Ju; Ji, Sumin; Ko, Junsu; Kim, Seong-Jin; Ryu, Hojin; Hong, Chang Pyo
Issue Date
2019-02-08
Publisher
BioMed Central
Citation
BMC Genomics. 2019 Feb 08;20(1):121
Keywords
Brown filmCell wall degradationFruit bodyLentinula edodesLight sensingMyceliumTranscriptome
Abstract
Background
Lentinula edodes is one of the most popular edible mushroom species in the world and contains useful medicinal components, such as lentinan. The light-induced formation of brown film on the vegetative mycelial tissues of L. edodes is an important process for ensuring the quantity and quality of this edible mushroom. To understand the molecular mechanisms underlying this critical developmental process in L. edodes, we characterized the morphological phenotypic changes in a strain, Chamaram, associated with abnormal brown film formation and compared its genome-wide transcriptional features.

Results
In the present study, we performed genome-wide transcriptome analyses of different vegetative mycelium growth phenotypes, namely, early white, normal brown, and defective dark yellow partial brown films phenotypes which were exposed to different light conditions. The analysis revealed the identification of clusters of genes specific to the light-induced brown film phenotypes. These genes were significantly associated with light sensing via photoreceptors such as FMN- and FAD-bindings, signal transduction by kinases and GPCRs, melanogenesis via activation of tyrosinases, and cell wall degradation by glucanases, chitinases, and laccases, which suggests these processes are involved in the formation of mycelial browning in L. edodes. Interestingly, hydrophobin genes such as SC1 and SC3 exhibited divergent expression levels in the normal and abnormal brown mycelial films, indicating the ability of these genes to act in fruiting body initiation and formation of dikaryotic mycelia. Furthermore, we identified the up-regulation of glycoside hydrolase domain-containing genes in the normal brown film but not in the abnormal film phenotype, suggesting that cell wall degradation in the normal brown film phenotype is crucial in the developmental processes related to the initiation and formation of fruiting bodies.

Conclusions
This study systematically analysed the expression patterns of light-induced browning-related genes in L. edodes. Our findings provide information for further investigations of browning formation mechanisms in L. edodes and a foundation for future L. edodes breeding.
ISSN
1471-2164
Language
English
URI
http://hdl.handle.net/10371/147175
DOI
https://doi.org/10.1186/s12864-019-5509-4
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Graduate School of Convergence Science and Technology (융합과학기술대학원)Dept. of Transdisciplinary Studies(융합과학부)Journal Papers (저널논문_융합과학부)
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