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Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jun Ho | - |
dc.contributor.author | Heo, Jeong-Hwa | - |
dc.contributor.author | Chang, Sun O | - |
dc.contributor.author | Kim, Chong-Sun | - |
dc.contributor.author | Oh, Seung-Ha | - |
dc.date.accessioned | 2009-11-24T23:55:20Z | - |
dc.date.available | 2009-11-24T23:55:20Z | - |
dc.date.issued | 2006-07-15 | - |
dc.identifier.citation | Hear Res. 2006 Sep;219(1-2):66-73. Epub 2006 Jul 12. | en |
dc.identifier.issn | 0378-5955 (Print) | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T73-4KCXJF1-6&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=cf4ad093796809579b070a3b08a29290 | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16839719 | - |
dc.identifier.uri | https://hdl.handle.net/10371/14775 | - |
dc.description.abstract | Extracellular ATP decreases K+ secretion in strial marginal cells via apical P2Y4 receptors. We investigated the effect of reactive blue 2 (RB-2), an antagonist of rat P2Y4, on rat strial marginal cells using a voltage-sensitive vibrating probe. The application of RB-2 increased K+ secretion in a dose-dependent manner, and this increase was characterized as a peak followed by a partial relaxation to a steady-state. Moreover, this response was similar to that caused by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Suramin had no similar effect, except at high concentration. Thus, we tested the effects of these chemicals on P2Y4 receptors in strial marginal cells. Both RB-2 and DIDS had antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion. Interestingly, 2'- and 3'-O-(4-benzoyl-benzoyl)adenosine 5'-triphosphate (BzATP) exhibited an agonistic effect at P2Y4 receptor, which was blocked by RB-2, but not by pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS). Based on these results, we speculate that direct and/or indirect inhibitory mechanisms between P2Y4 and KENQ1/KCNE1 K+ channels exist in strial marginal cell. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology | en |
dc.subject | Adenosine Triphosphate/analogs & derivatives/pharmacology | en |
dc.subject | Animals | en |
dc.subject | Dose-Response Relationship, Drug | en |
dc.subject | Electrophysiology | en |
dc.subject | Enzyme Inhibitors/*pharmacology | en |
dc.subject | Ion Transport/drug effects/physiology | en |
dc.subject | Perilymph/metabolism | en |
dc.subject | Potassium/*metabolism | en |
dc.subject | Rats | en |
dc.subject | Receptors, Purinergic P2/agonists/*antagonists & inhibitors | en |
dc.subject | Sodium-Potassium-Exchanging ATPase/metabolism | en |
dc.subject | Stria Vascularis/cytology/*drug effects/secretion | en |
dc.subject | Suramin/pharmacology | en |
dc.subject | Triazines/*pharmacology | en |
dc.subject | Uridine Triphosphate/pharmacology | en |
dc.subject | Vibration | en |
dc.title | Reactive blue 2, an antagonist of rat P2Y4, increases K+ secretion in rat cochlea strial marginal cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이준호 | - |
dc.contributor.AlternativeAuthor | 허정화 | - |
dc.contributor.AlternativeAuthor | 장선오 | - |
dc.contributor.AlternativeAuthor | 김종선 | - |
dc.contributor.AlternativeAuthor | 오승하 | - |
dc.identifier.doi | 10.1016/j.heares.2006.05.011 | - |
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