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Production of steviol from steviol glucosides using beta-glucosidase from a commercial pectinase, Sumizyme PX
DC Field | Value | Language |
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dc.contributor.author | Nguyen, Thi Thanh Hanh | - |
dc.contributor.author | Seo, Changseop | - |
dc.contributor.author | Gu, Bon-Cheol | - |
dc.contributor.author | Lim, Hee-Jung | - |
dc.contributor.author | Ha, Jung-Min | - |
dc.contributor.author | Kim, Seong-Bo | - |
dc.contributor.author | Park, Jun-Seong | - |
dc.contributor.author | Kim, Doman | - |
dc.creator | 김도만 | - |
dc.date.accessioned | 2019-04-24T23:00:58Z | - |
dc.date.available | 2020-04-05T23:00:58Z | - |
dc.date.created | 2018-12-10 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | Biotechnology Letters, Vol.40 No.1, pp.197-204 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://hdl.handle.net/10371/148616 | - |
dc.description.abstract | To purify and characterize a specific enzyme from a commercial pectinase for the production of steviol from stevioside (Ste) without adding organic solvent and to improve steviol production. Commercial Sumizyme PX converted Ste to steviol with a yield of 98%. beta-Glucosidase from Sumizyme PX (beta glyPX) was purified in three steps with 12.5-fold purification and 51% yield. The specific activity of the purified beta glyPX was 141 U/mg. The molecular weight of beta glyPX was similar to 116 kDa on SDS-PAGE. Its optimum activity was at pH 3.5 and 65 A degrees C. It was stable for 12 h up to 55 A degrees C and for 24 h at pH 2-9.5. K (m) values of beta glyPX for pNPGal, oNPGlc, lactose, and Ste were 2.4, 0.7, 18, and 7.8 mM, respectively. The optimum conditions for steviol production were 55 A degrees C, 900 U/ml, 80 mg Ste/ml, 12 h. beta glyPX contains great potential for industrial steviol production from Ste. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Kluwer Academic Publishers | - |
dc.title | Production of steviol from steviol glucosides using beta-glucosidase from a commercial pectinase, Sumizyme PX | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s10529-017-2460-9 | - |
dc.citation.journaltitle | Biotechnology Letters | - |
dc.identifier.wosid | 000424228200025 | - |
dc.identifier.scopusid | 2-s2.0-85032017007 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201719167 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A079459 | - |
dc.description.srnd | CITE_RATE:1.846 | - |
dc.description.srnd | FILENAME:Production of steviol from steviol gluc식품독성 20 고부가가30 난수용 10 한일10 한인도 10탄한20.pdf | - |
dc.description.srnd | DEPT_NM:국제농업기술학과 | - |
dc.description.srnd | EMAIL:kimdm@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/e433f73e-d049-45e2-a9fd-8934e432f09b/link | - |
dc.citation.endpage | 204 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 197 | - |
dc.citation.volume | 40 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Doman | - |
dc.identifier.srnd | T201719167 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ASPERGILLUS-NIGER | - |
dc.subject.keywordPlus | SULFOLOBUS-SOLFATARICUS | - |
dc.subject.keywordPlus | GLYCOSIDIC SWEETENERS | - |
dc.subject.keywordPlus | HYDROLYSIS | - |
dc.subject.keywordPlus | RUBUSOSIDE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | GALACTOSIDASE | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | AGLYCONE | - |
dc.subject.keywordAuthor | beta-Glucosidase | - |
dc.subject.keywordAuthor | Steviol | - |
dc.subject.keywordAuthor | Stevioside | - |
dc.subject.keywordAuthor | Sumizyme PX | - |
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