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Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo rohita macrophages via the suppression of the NF-κB signalling pathway : Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo rohita macrophages via the suppression of the NF-kappa B signalling pathway
DC Field | Value | Language |
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dc.contributor.author | Giri, Sib Sankar | - |
dc.contributor.author | Sen, Shib Sankar | - |
dc.contributor.author | Sukumaran, Venkatachalam | - |
dc.contributor.author | Park, Se Chang | - |
dc.creator | 박세창 | - |
dc.date.accessioned | 2019-04-25T00:12:42Z | - |
dc.date.available | 2021-03-24T11:26:23Z | - |
dc.date.created | 2018-09-07 | - |
dc.date.created | 2018-09-07 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | Fish and Shellfish Immunology, Vol.56, pp.459-466 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://hdl.handle.net/10371/148868 | - |
dc.description.abstract | Pinocembrin is a flavonoid that has been reported to exhibit various pharmacological and biological activities including antimicrobial, antioxidant, and anti-inflammatory. To explore the anti-inflammatory activity of pinocembrin in a fish cell line, we investigated its ability to regulate the inflammatory mediators elevated by lipopolysaccharide (LPS) in Labeo rohita head-kidney (HK) macrophages. HK macrophages of L. rohita were treated with LPS (1 (mu g mL(-1)) in the presence or absence of pinocembrin. We examined the inhibitory effect of pinocembrin on LPS-induced nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production. The inhibitory effect of pinocembrin on nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was investigated by RT-PCR and western blot. The effect of pinocembrin on pro-inflammatory cytokines (tumour necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta)) and anti-inflammatory cytokine IL-10 was investigated by ELISA and RT-PCR. The phosphorylation of three mitogen activated protein kinases (MAPKs) ERK, JNK, and p38 was analysed by western blot. Pinocembrin inhibited LPS-induced productions of NO and PGE2, and also markedly inhibited TNF-alpha, IL-1 beta, iNOS, and COX-2 production in a concentration-dependent manner. In addition, TNF-alpha and IL-1 beta mRNA expression levels decreased significantly, while IL-10 mRNA expression increased (P < 0.05) with pinocembrin pre-treatment. RT-PCR and western blot analysis showed that pinocembrin decreased both the mRNA and protein expression levels of LPS-induced iNOS and COX-2 in HK macrophages. Pinocembrin suppressed the phosphorylation of MAPK in LPS-stimulated HK macrophages. Further, pinocembrin significantly inhibited LPS-induced NF-kappa B transcriptional activity via the attenuation of I kappa B alpha degradation. Taken together, pinocembrin reduced the levels of pro-inflammatory mediators, such as iNOS, COX-2, TNF-alpha, and IL-1 beta, by inhibiting NF-kappa B activation via the suppression of ERK and p38 phosphorylation, and by attenuating the degradation of I kappa B alpha. These results suggest that pinocembrin is a potential novel candidate for the treatment of inflammatory conditions in L. rohita macrophages. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Academic Press | - |
dc.title | Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo rohita macrophages via the suppression of the NF-κB signalling pathway | - |
dc.title.alternative | Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo rohita macrophages via the suppression of the NF-kappa B signalling pathway | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fsi.2016.07.038 | - |
dc.citation.journaltitle | Fish and Shellfish Immunology | - |
dc.identifier.wosid | 000383292200050 | - |
dc.identifier.scopusid | 2-s2.0-84982179082 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201600579 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A076079 | - |
dc.description.srnd | CITE_RATE:3.148 | - |
dc.description.srnd | DEPT_NM:수의학과 | - |
dc.description.srnd | EMAIL:parksec@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.citation.endpage | 466 | - |
dc.citation.startpage | 459 | - |
dc.citation.volume | 56 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Se Chang | - |
dc.identifier.srnd | T201600579 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CYTOKINE GENE-EXPRESSION | - |
dc.subject.keywordPlus | HEAD-KIDNEY MACROPHAGES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ENDOTOXIC-SHOCK | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FISH | - |
dc.subject.keywordPlus | LPS | - |
dc.subject.keywordPlus | KINASES | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordAuthor | Labeo rohita macrophages | - |
dc.subject.keywordAuthor | Lipopolysaccharide | - |
dc.subject.keywordAuthor | Pinocembrin | - |
dc.subject.keywordAuthor | NF-kappa B signalling pathway | - |
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