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Identification and characterization of a novel cancer/testis antigen gene CAGE

Cited 68 time in Web of Science Cited 80 time in Scopus
Authors
Cho, Bomsoo; Lim, Yoon; Lee, Dae-Yeon; Park, Sae-Young; Lee, Hosoon; Kim, Woo Ho; Yang, Hankwang; Bang, Yung-Jue; Jeoung, Doo-Il
Issue Date
2002-04-02
Publisher
Elsevier
Citation
Biochem Biophys Res Commun. 2002 Apr 5;292(3):715-26.
Keywords
Adenoma/*genetics/immunologyAmino Acid SequenceAnimalsAntigens, Neoplasm/*genetics/immunology/metabolismAntigens, NuclearBase SequenceCarcinoma/*genetics/immunologyCell Cycle/physiologyCricetinaeDEAD-box RNA HelicasesFemaleGene Expression Regulation, Neoplastic/physiologyHumansMaleMiddle AgedMolecular Sequence DataNeoplasm Proteins/*genetics/immunology/metabolismNuclear Proteins/chemistry/*genetics/immunology/metabolismSequence AlignmentStomach Neoplasms/*genetics/immunologyTestis/chemistry/*immunology/metabolismTissue DistributionTumor Cells, CulturedX Chromosome
Abstract
We applied serological analysis of cDNA expression library technique to identify cancer-associated genes. We screened cDNA expression libraries of human testis and gastric cancer cell lines with sera of patients with gastric cancers. We identified a gene whose expression is testis-specific among normal tissues. We cloned and characterized this novel gene. It contains D-E-A-D box domain and encodes a putative protein of 630 amino acids with possible helicase activity. It showed wide expression in various cancer tissues and cancer cell lines. The corresponding gene was named cancer-associated gene (CAGE). PCR of human x hamster Radiation Hybrids showed localization of CAGE on the human chromosome Xp22. Transient transfection of CAGE showed predominantly nuclear localization. Both Western blot and plaque assay indicated seroreactivity of CAGE protein. We found that demethylation played a role in the activation of CAGE in some cancer cell lines that do not express it. Cell synchronization experiments showed that the expression of CAGE was related with cell cycle. This suggests that CAGE might play a role in cellular proliferation. Because CAGE is expressed in a variety of cancers but not in normal tissues except testis, this gene can be a target of antitumor immunotherapy.
ISSN
0006-291X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11922625

http://hdl.handle.net/10371/14996
DOI
https://doi.org/10.1006/bbrc.2002.6701
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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