Application of high throughput cell array technology to FISH: investigation of the role of deletion of p16 gene in leukemias

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Lee, Dong Soon; Lee, Jee Hyung; Min, Hyun Chung; Kim, Tae Young; Oh, Bo Ra; Kim, Ho Young; Lee, Jong Yun; Lee, Chung Kee; Chun, Hong Gu; Kim, Hee Chan
Issue Date
J Biotechnol. 2007 Jan 10;127(3):355-60. Epub 2006 Jul 29.
Acute DiseaseChronic DiseaseCytogenetic Analysis/methodsFemale*Gene Deletion*Genes, p16Humans*In Situ Hybridization, FluorescenceLeukemia/diagnosis/*geneticsMale*Microarray AnalysisMultiple Myeloma/diagnosis/*geneticsPredictive Value of Tests
Bio-cell chip is a chip that has hundreds of types of cells arrayed and immobilized on a small slide. To elucidate the role of deletion of the p16 gene in hematologic malignancies, the bio-cell chip technique was applied to fluorescent in situ hybridization (FISH) study. We made a bio-cell chip with bone marrow specimen from 109 patients with acute lymphoblastic leukemia (ALL), 102 patients with acute myelogenous leukemia (AML), 47 patients with chronic myelogenous leukemia (CML), and 25 patients with multiple myeloma (MM). A glass slide with 96 separated areas was fabricated, onto which was added methanol/acetic acid fixed cell suspensions for high-throughput FISH for p16. With the successful application of bio-cell chip technique, we found that the deletion of p16 contributed to the oncogenesis in acute leukemia, but not in chronic leukemia. In conclusion, the bio-cell chip, a cell version of ultrahigh-throughput technology, was successfully applied to the FISH study, which can be utilized efficiently in the molecular cytogenetic investigation of hematologic malignancies.
0168-1656 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Biomedical Engineering (의공학전공)Journal Papers (저널논문_의공학전공)
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