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Induction method of tyrosine kinase A-mediated cell death in rat pheochromocytoma

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Authors
Ahn, Jin-Young; Kang, Lami; Kim, Hyun-Jeong; Kang, Hyejin; Kim, Sung-Hoon; Kim, Je-Yong; Kim, Seung Ki; Wang, Kyu-Chang; Cho, Byung Kyu; Lee, Kwang-Woo; Roh, Jae-Kyu; Kim, Manho
Issue Date
2005-05-24
Publisher
Springer Verlag
Citation
Biotechnol Lett. 2005 Apr;27(8):583-7.
Keywords
AnimalsApoptosis/*drug effectsBlotting, WesternCarbazoles/pharmacologyCell Survival/drug effectsCulture Media, Serum-Free/pharmacologyEnzyme Inhibitors/pharmacologyIndole AlkaloidsNerve Growth Factor/*pharmacologyPC12 CellsPheochromocytoma/metabolism/pathologyRatsReceptor, trkA/antagonists & inhibitors/*metabolism
Abstract
Nerve Growth Factor (NGF) is a neurotrophic factor that prevents apoptosis in neuronal progenitor cells. In rat pheochromocytoma (PC12) cells, tyrosine kinase A receptor (TrkA) mediates neurotrophic or protective effects, while p75 neurotrophin receptor (p75NTR) functions as a death receptor. We have determined whether TrkA mediates any cytotoxic effect. Following serum deprivation, TrkA expression increased 2.2-fold and apoptosis began with expression of Bax proapoptotic protein. Application of NGF halved cell viability but this was reversed by K252a, the TrkA inhibitor. These results confirmed the paradoxical cytotoxic effect of neurotrophic NGF via TrkA in PC12 cells following serum deprivation.
ISSN
0141-5492 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15973493

http://hdl.handle.net/10371/15558
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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