S-Space College of Medicine/School of Medicine (의과대학/대학원) Laboratory Medicine (검사의학전공) Journal Papers (저널논문_검사의학전공)
Dissemination of SHV-12 and CTX-M-type extended-spectrum beta-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea
- Ryoo, Nam Hee; Kim, Eui-Chong; Hong, Seong Geun; Park, Yeon Joon; Lee, Kyungwon; Bae, Il Kwon; Song, Eun Hyang; Jeong, Seok Hoon
- Issue Date
- Oxford University Press
- J Antimicrob Chemother. 2005 Oct;56(4):698-702. Epub 2005 Sep 2.
- Anti-Bacterial Agents/pharmacology; Drug Resistance, Bacterial; Escherichia coli Infections/epidemiology/*microbiology; Hospitals; Humans; Klebsiella Infections/epidemiology/*microbiology; Korea/epidemiology; Escherichia coli/drug effects/*enzymology/genetics/isolation & purification; Klebsiella pneumoniae/drug effects/*enzymology/genetics/isolation & purification; Microbial Sensitivity Tests; beta-Lactamases/*genetics
- OBJECTIVES: To assess the prevalence and genotypes of Ambler class A extended-spectrum beta-lactamases (ESBLs) in Korea. METHODS: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February-July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A beta-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. RESULTS: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously. CONCLUSIONS: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
- 0305-7453 (Print)
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