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Effect of glycine on recovery of bladder smooth muscle contractility after acute urinary retention in rats

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors
Hong, Sung K; Son, Hwancheol; Kim, Soo W; Oh, Seung-June; Choi, Hwang
Issue Date
2005-11-17
Publisher
Blackwell Publishing
Citation
BJU Int. 2005 Dec;96(9):1403-8.
Keywords
AnimalsApoptosis/drug effectsBlotting, WesternFemaleGlycine/*pharmacologyGlycine Agents/*pharmacologyMuscle Contraction/*drug effectsMuscle, Smooth/*drug effectsProto-Oncogene Proteins c-bcl-2/metabolismRatsRats, Sprague-DawleyUrinary Bladder/*drug effectsUrinary Retention/*physiopathology
Abstract
OBJECTIVE: To investigate the effects of glycine on the recovery of bladder smooth muscle contractility after acute urinary retention. MATERIALS AND METHODS: Bladder overdistension was induced in Sprague-Dawley rats by an infusion of saline (twice the threshold volume), maintained for 2 h. From 15 min before emptying of the bladder until 2 h after, saline or glycine solution was infused i.v. At 30 min, 2 h and 1 week after bladder emptying, samples of bladder tissue were taken for muscle strip study, malondialdehyde (MDA) assay, ATP assay, Western blotting for apoptosis-related molecules (Bcl-2, Bax, Caspase-3), and histological analysis including terminal deoxynucleotidyl transferase-mediated nick-end labelling staining. The results were compared among normal control, saline-treated and glycine-treated rats. RESULTS: In the glycine-treated group, muscle strip contractile responses induced by electrical-field stimulation and carbachol were both significantly greater at 1 week after bladder emptying than in the saline-treated group. The results of the ATP assay appeared to correspond with those of the muscle strip study. The saline-treated group had significantly higher MDA levels at 30 min after bladder emptying than the glycine-treated group. At 2 h after bladder emptying, there was significantly more apoptosis and greater leukocyte infiltration in the saline-treated group than in the glycine-treated group. While pro-apoptotic Bax and caspase-3 were down-regulated, Bcl-2 was up-regulated in the glycine-treated group. CONCLUSION: Glycine infusions might improve the contractile responses of bladder smooth muscle after acute urinary retention by reducing oxidative damage and apoptosis.
ISSN
1464-4096 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16287466

http://hdl.handle.net/10371/15719
DOI
https://doi.org/10.1111/j.1464-410X.2005.05855.x
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College of Medicine/School of Medicine (의과대학/대학원)Urology (비뇨기과학전공)Journal Papers (저널논문_비뇨기과학전공)
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