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Intravenous administration of human neural stem cells induces functional recovery in Huntington's disease rat model

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Authors
Lee, Soon-Tae; Chu, Kon; Park, Jung-Eun; Lee, Kyungmi; Kang, Lami; Kim, Seung U; Kim, Manho
Issue Date
2005-05-18
Publisher
Elsevier
Citation
Neurosci Res. 2005 Jul;52(3):243-9.
Keywords
AnimalsApomorphine/pharmacologyBehavior, Animal/drug effects/physiologyCell Count/methodsCells, CulturedCorpus Striatum/drug effects/metabolism/pathologyDNA-Binding Proteins/metabolismDisease Models, AnimalDopamine and cAMP-Regulated Phosphoprotein 32Drosophila Proteins/metabolismEndogenous Retroviruses/geneticsFunctional LateralityGalactosides/diagnostic useGlial Fibrillary Acidic Protein/metabolismHumansHuntington Disease/chemically induced/*therapyImmunohistochemistry/methodsIndoles/diagnostic useInfusions, Intravenous/*methodsMaleMotor Activity/drug effects/physiologyNerve Tissue Proteins/metabolismNeurons/*physiologyParvalbumins/metabolismPhosphoproteins/metabolismPhosphopyruvate Hydratase/metabolismQuinolinic AcidRNA, Messenger/biosynthesisRandom AllocationRatsRats, Sprague-DawleyRecovery of Function/*physiologyReverse Transcriptase Polymerase Chain Reaction/methodsRotarod Performance Test/methodsStem Cell Transplantation/*methodsStem Cells/*physiologyTime Factorsgamma-Aminobutyric Acid/metabolism
Abstract
An animal model induced by striatal quinolinic acid (QA) injection shows ongoing striatal degeneration mimicking Huntington's disease. To study the migratory ability and the neuroprotective effect of human neural stem cells (NSCs) in this model, we transplanted NSCs (5 x 10(6)) or saline intravenously at 7 days after unilateral QA injection. NSCs-group exhibited the reduced apomorphine-induced rotation and the reduced striatal atrophy compared to the control. PCR analysis for the human-specific ERV-3 gene supported an evidence of the engraftment of human NSCs in the rat brain. X-gal+ cells were found in and around the damaged striatum and migrated NSCs differentiated into neurons and glias. This result indicates that intravenously injected human NSCs can migrate into the striatal lesion, decrease the following striatal atrophy, and induce long-term functional improvement in a glutamate toxicity-induced striatal degeneration model.
ISSN
0168-0102 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15896865

http://hdl.handle.net/10371/15815
DOI
https://doi.org/10.1016/j.neures.2005.03.016
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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