Browse
S-Space
College of Medicine/School of Medicine (의과대학/대학원)
Surgery (외과학전공)
Journal Papers (저널논문_외과학전공)
BetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast cancer
- Issue Date
- 2004-12-15
- Publisher
- John Wiley & Sons
- Citation
- J Cell Biochem. 2005 Apr 1;94(5):1010-6
- Keywords
- Animals ; Blotting, Western ; Breast Neoplasms/*enzymology/pathology ; Cell Cycle Proteins/*physiology ; Female ; Guanine Nucleotide Exchange Factors/*physiology ; Humans ; Hydrolysis ; Immunohistochemistry ; Mice ; NIH 3T3 Cells ; Phospholipase C gamma ; Protein Binding ; Type C Phospholipases/*metabolism ; src Homology Domains
- Abstract
- Phospholipase C-gamma1 (PLCgamma1) plays a critical role in cell growth and proliferation by generating the second messengers, diacylglycerol and 1, 4, 5-inositol triphosphate. To investigate the roles of Src homology domain 2 and domain 3 of PLCgamma1 in PLCgamma1-mediated cell signaling, we characterized some proteins binding to these domains in the MCF7 and MDA-MB-231 breast cancer cell lines. Of the several proteins that bind to glutathione-S-transferase-SH2/SH2/SH3, we identified an 85 kDa protein that binds to the SH3 domain of PLCgamma1 as the guanine nucleotide exchange factor, p21-activated protein kinase-interacting exchange factor-a (betaPix-a). BetaPix-a co-immunoprecipitated with PLCgamma1 in breast cancer tissues extracts and in MCF7 and MDA-MB-231 cell extracts. In addition, PDGF-stimulated PLCgamma1 activity was elevated in betaPix-a-overexpressing NIH3T3 cells. Our results suggest that betaPix-a binds to the Src homology domain 3 of PLCgamma1 and promotes tumor growth in breast cancer by enhancing the activity PLCgamma1.
- ISSN
- 0730-2312 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15597340
https://hdl.handle.net/10371/15821
- Files in This Item: There are no files associated with this item.
- Appears in Collections:
- College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.