SHERP

BetaPix-a enhances the activity of phospholipase Cgamma1 by binding SH3 domain in breast cancer

Cited 0 time in webofscience Cited 6 time in scopus
Authors
Bae, Ji-Yeon; Ahn, Soo-Jung; Lee, Jeong Eon; Kim, Jung-Eun; Han, Mi-Ryung; Han, Wonshik; Kim, Seok Won; Shin, Hyuk Jai; Lee, Seung Joon; Park, Dongeun; Noh, Dong-Young
Issue Date
2004-12-15
Publisher
John Wiley & Sons
Citation
J Cell Biochem. 2005 Apr 1;94(5):1010-6
Keywords
AnimalsBlotting, WesternBreast Neoplasms/*enzymology/pathologyCell Cycle Proteins/*physiologyFemaleGuanine Nucleotide Exchange Factors/*physiologyHumansHydrolysisImmunohistochemistryMiceNIH 3T3 CellsPhospholipase C gammaProtein BindingType C Phospholipases/*metabolismsrc Homology Domains
Abstract
Phospholipase C-gamma1 (PLCgamma1) plays a critical role in cell growth and proliferation by generating the second messengers, diacylglycerol and 1, 4, 5-inositol triphosphate. To investigate the roles of Src homology domain 2 and domain 3 of PLCgamma1 in PLCgamma1-mediated cell signaling, we characterized some proteins binding to these domains in the MCF7 and MDA-MB-231 breast cancer cell lines. Of the several proteins that bind to glutathione-S-transferase-SH2/SH2/SH3, we identified an 85 kDa protein that binds to the SH3 domain of PLCgamma1 as the guanine nucleotide exchange factor, p21-activated protein kinase-interacting exchange factor-a (betaPix-a). BetaPix-a co-immunoprecipitated with PLCgamma1 in breast cancer tissues extracts and in MCF7 and MDA-MB-231 cell extracts. In addition, PDGF-stimulated PLCgamma1 activity was elevated in betaPix-a-overexpressing NIH3T3 cells. Our results suggest that betaPix-a binds to the Src homology domain 3 of PLCgamma1 and promotes tumor growth in breast cancer by enhancing the activity PLCgamma1.
ISSN
0730-2312 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15597340

http://hdl.handle.net/10371/15821
DOI
https://doi.org/10.1002/jcb.20357
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse