Presence of CCK-A, B receptors and effect of gastrin and cholecystokinin on growth of pancreatobiliary cancer cell lines

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Jang, Jin-Young; Kim, Sun-Whe; Ku, Ja-Lok; Park, Yong-Hyun; Park, Jae-Gahb
Issue Date
World J Gastroenterol. 2005 Feb 14;11(6):803-9
Biliary Tract Neoplasms/metabolism/*physiopathologyCell CountCell Division/drug effectsCell Line, TumorCholecystokinin/metabolism/*pharmacologyGastrins/metabolism/*pharmacologyHormone Antagonists/pharmacologyHumansPancreatic Neoplasms/metabolism/*physiopathologyReceptor, Cholecystokinin A/*genetics/metabolismReceptor, Cholecystokinin B/*genetics/metabolismReverse Transcriptase Polymerase Chain ReactionTetrazolium SaltsThiazoles
AIM: To investigate the effects of gastrin and cholecystokinin (CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell lines. METHODS: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell lines. RESULTS: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor mRNA. CONCLUSION: Gastrin and CCK exert a trophic action on some of the biliary tract cancers.
1007-9327 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Surgery (외과학전공)Journal Papers (저널논문_외과학전공)
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