Combined low dose cyclosporine and prednisone down-regulate natural killer cell-like effector functions of CD8brightCD56+ T cells in patients with active Behcet uveitis

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Ahn, Jae Kyoun; Park, Yeoung Geol; Park, Sang Woo; Yoon, Kyung-Chul; Yu, Hyeong Gon; Chung, Hum
Issue Date
Taylor & Francis
Ocul Immunol Inflamm. 2006 Oct;14(5):267-75.
AdultAntigens, CD56/*immunologyBehcet Syndrome/drug therapy/*immunologyCD8-Positive T-Lymphocytes/*physiologyCyclosporine/administration & dosageCytotoxicity Tests, ImmunologicDown-Regulation/*drug effectsDrug Therapy, CombinationFemaleFlow CytometryHumansImmunosuppressive Agents/*administration & dosageKiller Cells, Natural/*immunologyMaleMembrane Glycoproteins/metabolismMiddle AgedPerforinPhenotypePore Forming Cytotoxic Proteins/metabolismPrednisone/administration & dosageProspective StudiesReceptors, CCR5/metabolismReceptors, Interleukin-8A/metabolism
PURPOSE: To investigate the changes of effector-related phenotypic markers and the natural killer (NK)-like effector functions of CD8(bright)CD56+ T cells in patients with Behcet uveitis after combined cyclosporine and prednisone (Cs/Pd) treatment. METHODS: Ten patients with active Behcet panuveitis and 10 healthy controls were prospectively recruited in this study. The effector-related surface markers (CD27, CD62L, CD11b, HLA-DR, CD94, NKG2D), chemokine receptors (CXCR1, CXCR3, CCR4, CCR5), and intracellular perforin of circulating CD8(bright)CD56+ T cells were determined by flow cytometric analysis before and after two months' treatment. NK-like cytotoxicity of ex vivo CD8(bright)CD56+ T cells against K562 was measured by standard 51Cr release assay. RESULTS: The expression levels of effector-related molecules on CD8(bright)CD56+ T cells normalized after treatment. The expression levels of CXCR1 and CCR5 were down-regulated on CD8(bright)CD56+ T cells after treatment. The amounts of preformed intracellular perforin of CD8(bright)CD56+ T cells were reduced to the normal levels. Furthermore, the NK-like cytolytic capacities of CD8(bright)CD56+ T cells were decreased after treatment. CONCLUSIONS: Our results suggest that the combined Cs/Pd treatments in active Behcet uveitis may downregulate the NK-like effector functions of CD8(bright)CD56+ T cells.
0927-3948 (Print)
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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