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G-protein coupled receptor 64 (GPR64) acts as a tumor suppressor in endometrial cancer

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Authors
Ahn, Jong Il; Yoo, Jung-Yoon; Kim, Tae Hoon; Kim, Young Im; Broaddus, Russell R; Ahn, Ji Yeon; Lim, Jeong Mook; Jeong, Jae-Wook
Issue Date
2019-08-14
Publisher
BioMed Central
Citation
BMC Cancer, 19(1):810
Keywords
Endometrial cancerTumor suppressorGPR64Connexin 43
Abstract
Background
Endometrial cancer is the most common gynecological cancer. G-protein coupled receptor 64 (GPR64) belongs to a family of adhesion GPCRs and plays an important role in male fertility. However, the function of GPR64 has not been studied in endometrial cancer. Our objective is to investigate the role of GPR64 in endometrial cancer.

Methods
We examined the levels of GPR64 in human endometrioid endometrial carcinoma by immunohistochemistry analysis. To determine a tumor suppressor role of GPR64 in endometrial cancer, we used a siRNA loss of function approach in human endometrial adenocarcinoma cell lines.

Results
GPR64 levels were remarkably lower in 10 of 21 (47.62%) of endometrial carcinoma samples compared to control. Depletion of GPR64 by siRNA transfection revealed an increase of colony formation ability, cell proliferation, cell migration, and invasion activity in Ishikawa and HEC1A cells. The expression of Connexin 43 (Cx43), a member of the large family of gap junction proteins, was reduced through activation of AMP-activated protein kinase (AMPK) in Ishikawa cells with GPR64-deficicy.

Conclusions
These results suggest that GPR64 plays an important tumor suppressor role in endometrial cancer.
ISSN
1471-2407
Language
English
URI
http://hdl.handle.net/10371/162606
DOI
https://doi.org/10.1186/s12885-019-5998-1
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Journal Papers (저널논문_농생명공학부)
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