Hepatocyte growth factor suppresses vascular endothelial growth factor-induced expression of endothelial ICAM-1 and VCAM-1 by inhibiting the nuclear factor-kappaB pathway

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Min, Jeong-Ki; Lee, Young-Mi; Kim, Jeong Hun; Kim, Young-Myeong; Kim, Sung Wan; Lee, Soo-Young; Gho, Yong Song; Oh, Goo Taeg; Kwon, Young-Guen
Issue Date
American Heart Association
Circ Res. 2005 Feb 18;96(3):300-7. Epub 2005 Jan 6.
AnimalsCell Adhesion/physiologyCell Adhesion Molecules/biosynthesisCell LineCell Line, TumorChemotaxis, Leukocyte/physiologyEndothelial Cells/*metabolismEndothelium, Vascular/cytology/metabolismFemaleHepatocyte Growth Factor/*physiologyHumansI-kappa B KinaseIntercellular Adhesion Molecule-1/*biosynthesisInterleukin-1/physiologyLeukocytes/metabolismMiceMice, Inbred StrainsNF-kappa B/*antagonists & inhibitors/*metabolismPhosphorylationProtein-Serine-Threonine Kinases/metabolismRNA, Messenger/antagonists & inhibitors/geneticsTranscription, Genetic/physiologyTranscriptional Activation/physiologyTumor Necrosis Factor-alpha/physiologyU937 Cells/chemistry/metabolismUmbilical Veins/cytologyVascular Cell Adhesion Molecule-1/*biosynthesisVascular Endothelial Growth Factors/*antagonists & inhibitors/*physiology
Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) are potent angiogenic factors that have been used clinically to induce angiogenesis. However, concerns have been raised about VEGF because of its proinflammatory actions, which include enhancing the adhesion of leukocytes to endothelial cells. We have examined the possible antiinflammatory effects of HGF on the vasculature. HGF, unlike VEGF, did not alter leukocyte adhesion to endothelial cells. Instead it inhibited VEGF-induced leukocyte-endothelial cell interactions and the endothelial expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). In a skin inflammation model, VEGF-treated mice showed a significant increase of leukocytes infiltrated or adherent to the luminal surface of blood vessels, as compared with vehicle- or HGF-treated mice. The VEGF effect was markedly suppressed by coadministration of HGF. RT-PCR and promoter analysis revealed that HGF downregulated VEGF-mediated expression of ICAM-1 and VCAM-1 at the transcriptional level. Furthermore, these inhibitory effects coincided with suppression of IkappaB kinase activity, and this in turn prevented the activation of the inflammatory transcription factor NF-kappaB. Taken together, our results demonstrate that HGF suppresses VEGF-induced inflammation presumably by inhibiting the endothelial NF-kappaB pathway. This suggests that combined treatment with HGF and VEGF could be superior to treatment with either factor alone for enhancing therapeutic angiogenesis while avoiding inflammation.
1524-4571 (Electronic)
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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