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Catalytic activity of bovine seminal ribonuclease is essential for its immunosuppressive and other biological activities
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Soucek, Josef | - |
dc.contributor.author | Matousek, Josef | - |
dc.contributor.author | Raines, Ronald T. | - |
dc.date.accessioned | 2020-04-27T12:24:58Z | - |
dc.date.available | 2020-04-27T12:24:58Z | - |
dc.date.created | 2020-04-08 | - |
dc.date.created | 2020-04-08 | - |
dc.date.issued | 1995-06 | - |
dc.identifier.citation | Biochemical Journal, Vol.308, pp.547-550 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.other | 95225 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165589 | - |
dc.description.abstract | Bovine seminal ribonuclease (BS-RNase) is a homologue of RNase A with special biological properties, including potent immunosuppressive activity. A mutant BS-RNase was created in which His-119, the active-site residue that acts as a general acid during catalysis, was changed to an aspartic acid. H119D BS-RNase formed a dimer with quaternary structure similar to that of the wild-type enzyme but with values of k(cat) and k(cat.)/K-m for the cleavage of UpA [uridylyl(3' --> 5')adenosine] that were 4x10(3)-fold lower. The mutant protein also demonstrated dramatically decreased immunosuppressive, anti-tumour, aspermatogenic, and embryotoxic activities. The catalytic activity of BS-RNase is therefore necessary for its special biological properties. | - |
dc.language | 영어 | - |
dc.publisher | Portland Press, Ltd. | - |
dc.title | Catalytic activity of bovine seminal ribonuclease is essential for its immunosuppressive and other biological activities | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1042/bj3080547 | - |
dc.citation.journaltitle | Biochemical Journal | - |
dc.identifier.wosid | A1995RB32500026 | - |
dc.identifier.scopusid | 2-s2.0-0029006175 | - |
dc.citation.endpage | 550 | - |
dc.citation.startpage | 547 | - |
dc.citation.volume | 308 | - |
dc.identifier.sci | A1995RB32500026 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RNASE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ANGIOGENIN | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | GENE | - |
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