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Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels
DC Field | Value | Language |
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dc.contributor.author | Kwon, Heung-Sun | - |
dc.contributor.author | Shin, Hyun-Chul | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:29:42Z | - |
dc.date.available | 2020-04-27T12:29:42Z | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.issued | 2005-04 | - |
dc.identifier.citation | Nucleic Acids Research, Vol.33 No.8, p. e74 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.other | 93233 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165605 | - |
dc.description.abstract | Gene expression is regulated at the transcriptional and post-transcriptional levels. Therefore, in order to achieve a high level of silencing, which includes minimizing any residual expression of a target gene, suppression at both the transcriptional and post-transcriptional levels is required. In this study, we describe a new method for highly efficient gene silencing that combines zinc finger protein-mediated transcriptional repression and small interfering RNA (siRNA)-mediated inhibition of post-transcriptional events. To measure the amount of gene expression under various conditions, we used a luciferase reporter gene that was driven by a variety of promoters, including that of the human vascular endothelial growth factor-A (VEGF-A) gene. We also measured expression of the endogenous VEGF-A gene. Inhibition of gene expression by each of the two individual technologies was effective, but in-depth analyses revealed residual expression of the target gene. The combination of specific zinc finger transcription factors and siRNAs greatly enhanced the silencing of the human VEGF-A gene, not only when cells were grown in the presence of normal amounts of oxygen but also under conditions of hypoxic stimulation. These results suggest that a bi-level approach to the silencing of VEGF-A expression may be clinically beneficial as part of a cancer treatment protocol. | - |
dc.language | 영어 | - |
dc.publisher | Oxford University Press | - |
dc.title | Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1093/nar/gni068 | - |
dc.citation.journaltitle | Nucleic Acids Research | - |
dc.identifier.wosid | 000229113100005 | - |
dc.identifier.scopusid | 2-s2.0-27244436299 | - |
dc.citation.number | 8 | - |
dc.citation.startpage | e74 | - |
dc.citation.volume | 33 | - |
dc.identifier.sci | 000229113100005 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | DOUBLE-STRANDED-RNA | - |
dc.subject.keywordPlus | ZINC-FINGER PROTEINS | - |
dc.subject.keywordPlus | HIV-1 REPLICATION | - |
dc.subject.keywordPlus | INTERFERING RNAS | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | GENE-REGULATION | - |
dc.subject.keywordPlus | MOUSE OOCYTES | - |
dc.subject.keywordPlus | HUMAN GENOME | - |
dc.subject.keywordPlus | FACTOR-A | - |
dc.subject.keywordPlus | DESIGN | - |
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