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Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Seung Woo | - |
dc.contributor.author | Lee, Jihyun | - |
dc.contributor.author | Carroll, Dana | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Lee, Junho | - |
dc.date.accessioned | 2020-04-27T12:35:54Z | - |
dc.date.available | 2020-04-27T12:35:54Z | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.created | 2020-03-24 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.citation | Genetics, Vol.195 No.3, pp.1177-1180 | - |
dc.identifier.issn | 1943-2631 | - |
dc.identifier.other | 93184 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165626 | - |
dc.description.abstract | We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F-1 progeny, demonstrating the high efficiency of this method. | - |
dc.language | 영어 | - |
dc.publisher | Genetics Society Of America | - |
dc.title | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9-sgRNA Ribonucleoproteins | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.contributor.AlternativeAuthor | 이준호 | - |
dc.identifier.doi | 10.1534/genetics.113.155853 | - |
dc.citation.journaltitle | Genetics | - |
dc.identifier.wosid | 000329874100042 | - |
dc.identifier.scopusid | 2-s2.0-84884911076 | - |
dc.citation.endpage | 1180 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 1177 | - |
dc.citation.volume | 195 | - |
dc.identifier.sci | 000329874100042 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.contributor.affiliatedAuthor | Lee, Junho | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
dc.subject.keywordPlus | HUMAN GENOME | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | C. ELEGANS | - |
dc.subject.keywordPlus | CAS9 | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | UNC-1 | - |
dc.subject.keywordAuthor | Caenorhabditis elegans | - |
dc.subject.keywordAuthor | Cas9-sgRNA ribonucleoprotein | - |
dc.subject.keywordAuthor | NHEJ | - |
dc.subject.keywordAuthor | genome editing | - |
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