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RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Lee, Jeong-Soo | - |
| dc.contributor.author | Kwak, Su-Jin | - |
| dc.contributor.author | Kim, Jungeun | - |
| dc.contributor.author | Kim, Ae-Kyeong | - |
| dc.contributor.author | Noh, Hae Min | - |
| dc.contributor.author | Kim, Jin-Soo | - |
| dc.contributor.author | Yu, Kweon | - |
| dc.date.accessioned | 2020-04-27T12:37:54Z | - |
| dc.date.available | 2020-04-27T12:37:54Z | - |
| dc.date.created | 2020-03-23 | - |
| dc.date.created | 2020-03-23 | - |
| dc.date.issued | 2014-07 | - |
| dc.identifier.citation | G3: Genes, Genomes, Genetics, Vol.4 No.7, pp.1291-1295 | - |
| dc.identifier.issn | 2160-1836 | - |
| dc.identifier.other | 93163 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/165633 | - |
| dc.description.abstract | We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay. | - |
| dc.language | 영어 | - |
| dc.publisher | Genetics Society of America | - |
| dc.title | RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein | - |
| dc.type | Article | - |
| dc.contributor.AlternativeAuthor | 김진수 | - |
| dc.identifier.doi | 10.1534/g3.114.012179 | - |
| dc.citation.journaltitle | G3: Genes, Genomes, Genetics | - |
| dc.identifier.wosid | 000339326600010 | - |
| dc.identifier.scopusid | 2-s2.0-84904572233 | - |
| dc.citation.endpage | 1295 | - |
| dc.citation.number | 7 | - |
| dc.citation.startpage | 1291 | - |
| dc.citation.volume | 4 | - |
| dc.identifier.sci | 000339326600010 | - |
| dc.description.isOpenAccess | Y | - |
| dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
| dc.type.docType | Article | - |
| dc.description.journalClass | 1 | - |
| dc.subject.keywordPlus | GENE KNOCKOUT | - |
| dc.subject.keywordPlus | HUMAN-CELLS | - |
| dc.subject.keywordPlus | ENDONUCLEASES | - |
| dc.subject.keywordPlus | NUCLEASES | - |
| dc.subject.keywordPlus | CRISPR/CAS9 | - |
| dc.subject.keywordPlus | MICE | - |
| dc.subject.keywordAuthor | Cas9 protein | - |
| dc.subject.keywordAuthor | RGEN | - |
| dc.subject.keywordAuthor | Drosophila | - |
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