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Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations

Cited 65 time in Web of Science Cited 70 time in Scopus
Authors
Ramakrishna, Suresh; Cho, Seung Woo; Kim, Sojung; Song, Myungjae; Gopalappa, Ramu; Kim, Jin-Soo; Kim, Hyongbum
Issue Date
2014-02
Citation
Nature Communications, Vol.5, p. 3378
Abstract
RNA-guided endonucleases (RGENs), which are based on the clustered, regularly interspaced, short palindromic repeat (CRISPR)-CRISPR-associated (Cas) system, have recently emerged as a simple and efficient tool for genome editing. However, the activities of prepared RGENs are sometimes low, hampering the generation of cells containing RGEN-induced mutations. Here we report efficient methods to enrich cells containing RGEN-induced mutations by using surrogate reporters. HEK293T cells are cotransfected with the reporter plasmid, a plasmid encoding Cas9 and a plasmid encoding crRNA and tracrRNA, and subjected to flow cytometric sorting, magnetic separation or hygromycin selection. The selected cell populations are highly enriched with cells containing RGEN-induced mutations, by a factor of up to 11-fold as compared with the unselected population. The fold enrichment tends to be high when RGEN activity is low. We envision that these reporters will facilitate the use of RGEN in a wide range of biomedical research.
ISSN
2041-1723
URI
http://hdl.handle.net/10371/165639
DOI
https://doi.org/10.1038/ncomms4378
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