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Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koo, Taeyoung | - |
dc.contributor.author | Lee, Jungjoon | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2020-04-27T12:44:06Z | - |
dc.date.available | 2020-04-27T12:44:06Z | - |
dc.date.created | 2018-11-22 | - |
dc.date.created | 2018-11-22 | - |
dc.date.issued | 2015-06 | - |
dc.identifier.citation | Molecules and Cells, Vol.38 No.6, pp.475-481 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | 69680 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165653 | - |
dc.description.abstract | Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations. | - |
dc.language | 영어 | - |
dc.publisher | 한국분자세포생물학회 | - |
dc.title | Measuring and reducing off-target activities of programmable nucleases including CRISPR-Cas9 | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.14348/molcells.2015.0103 | - |
dc.citation.journaltitle | Molecules and Cells | - |
dc.identifier.wosid | 000358893700001 | - |
dc.identifier.scopusid | 2-s2.0-84938739634 | - |
dc.citation.endpage | 481 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 475 | - |
dc.citation.volume | 38 | - |
dc.identifier.sci | 000358893700001 | - |
dc.identifier.kciid | ART001997885 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ZINC-FINGER NUCLEASES | - |
dc.subject.keywordPlus | RNA-GUIDED ENDONUCLEASES | - |
dc.subject.keywordPlus | GENOME-WIDE ANALYSIS | - |
dc.subject.keywordPlus | HUMAN-CELLS | - |
dc.subject.keywordPlus | ENGINEERED NUCLEASES | - |
dc.subject.keywordPlus | CRISPR/CAS9 SYSTEMS | - |
dc.subject.keywordPlus | GENE CORRECTION | - |
dc.subject.keywordPlus | CAS NUCLEASES | - |
dc.subject.keywordPlus | DNA CLEAVAGE | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordAuthor | Cas9 | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | genome editing | - |
dc.subject.keywordAuthor | off-target | - |
dc.subject.keywordAuthor | TALEN | - |
dc.subject.keywordAuthor | ZFN | - |
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