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Structural insights into the apo-structure of Cpf1 protein from Francisella novicida
DC Field | Value | Language |
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dc.contributor.author | Min, Kyungjin | - |
dc.contributor.author | Yoon, Hyunjun | - |
dc.contributor.author | Jo, Inseong | - |
dc.contributor.author | Ha, Nam-Chul | - |
dc.contributor.author | Jin, Kyeong Sik | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Lee, Hyung Ho | - |
dc.date.accessioned | 2020-04-27T13:00:31Z | - |
dc.date.available | 2020-04-27T13:00:31Z | - |
dc.date.created | 2018-12-31 | - |
dc.date.created | 2018-12-31 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, Vol.498 No.4, pp.775-781 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.other | 71946 | - |
dc.identifier.uri | https://hdl.handle.net/10371/165705 | - |
dc.description.abstract | Clustered regularly interspaced short palindromic repeats (CRISPRs) from Prevotella and Francisella (Cpf1) are RNA-guided endonucleases that produce cohesive double-stranded breaks in DNA by specifically recognizing thymidine-rich protospacer-adjacent motif (PAM) sequences. Cpf1 is emerging as a powerful genome-editing tool. Despite previous structural studies on various Cpf1 proteins, the apo-structure of Cpf1 remains unknown. In the present study, we determined the solution structure of the Cpf1 protein from Francisella novicida (FnCpf1) with and without CRISPR RNA (crRNA) using small-angle X-ray scattering, providing the insights into the apo-structure of FnCpf1. The apo-structure of FnCpf1 was also visualized using negative staining electron microcopy. When we compared the apo-structure of FnCpf1 with crRNA-bound structure, their overall shapes (a closed form) were similar, suggesting that conformational change upon crRNA binding to FnCpf1 is not drastic, but a local induced fit might occur to recognize PAM sequences. In contrast, the apo Cpf1 from Moraxella bovoculi 237 (MbCpf1) was analyzed as an open form, implying that a large conformational change from an open to a closed form might be required for crRNA binding to MbCpf1. These results suggested that the crRNA-induced conformational changes in Cpf1 differ among species. (C) 2018 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Structural insights into the apo-structure of Cpf1 protein from Francisella novicida | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.contributor.AlternativeAuthor | 이형호 | - |
dc.contributor.AlternativeAuthor | 하남출 | - |
dc.identifier.doi | 10.1016/j.bbrc.2018.03.057 | - |
dc.citation.journaltitle | Biochemical and Biophysical Research Communications | - |
dc.identifier.wosid | 000430158200012 | - |
dc.identifier.scopusid | 2-s2.0-85043371501 | - |
dc.citation.endpage | 781 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 775 | - |
dc.citation.volume | 498 | - |
dc.identifier.sci | 000430158200012 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Ha, Nam-Chul | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Ho | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | RAY SOLUTION SCATTERING | - |
dc.subject.keywordPlus | SMALL-ANGLE SCATTERING | - |
dc.subject.keywordPlus | CRISPR-CAS SYSTEMS | - |
dc.subject.keywordPlus | TARGET DNA | - |
dc.subject.keywordPlus | PAM RECOGNITION | - |
dc.subject.keywordPlus | GUIDE RNA | - |
dc.subject.keywordPlus | DUAL-RNA | - |
dc.subject.keywordPlus | ENDONUCLEASE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordAuthor | Cpf1 | - |
dc.subject.keywordAuthor | CRISPR-Cas system | - |
dc.subject.keywordAuthor | Genome-editing | - |
dc.subject.keywordAuthor | SAXS | - |
dc.subject.keywordAuthor | Electron microscopy | - |
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