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Characterization of microbiome in bronchoalveolar lavage fluid of patients with lung cancer comparing with benign mass like lesions

Cited 48 time in Web of Science Cited 47 time in Scopus
Authors
Lee, Sang Hoon; Sung, Ji Yeon; Yong, Dongeun; Chun, Jongsik; Kim, Song Yee; Song, Joo Han; Chung, Kyung Soo; Kim, Eun Young; Jung, Ji Ye; Kang, Young Ae; Kim, Young Sam; Kim, Se Kyu; Chang, Joon; Park, Moo Suk
Issue Date
2016-12
Citation
Lung Cancer, Vol.102, pp.89-95
Keywords
Lung cancerBenign massBronchoalveolar fluidMicrobiomeNew generation sequencing
Abstract
Objectives: Disruption in the stability of respiratory microbiota is known to be associated with many chronic respiratory diseases. However, only few studies have examined microbiomes in lung cancer. Therefore, we characterized and compared the microbiomes of patients with lung cancer and those with benign mass-like lesions. Materials and methods: Bronchoalveolar fluid was collected prospectively to evaluate lung masses in patients who had undergone bronchoscopies from May to September 2015. Twenty-eight patients (20 male, 8 female) were enrolled: 20 diagnosed with lung cancer and 8 diagnosed with benign diseases. Samples were analysed by 165 rRNA-based next-generation sequencing. Results: The participants' mean age was 64 +/- 11 years. Bacterial operational taxonomic units were classified into 26 phyla, 44 classes, 81 orders, 153 families, 288 genera, and 797 species. The relative abundance of two phyla (Firmicutes and TM7) was significantly increased in patients with lung cancer (p = 0.037 and 0.035, respectively). Furthermore, two genera (Veillonella and Megasphaera) were relatively more abundant in lung cancer patients (p = 0.003 and 0.022, respectively). The area under the curve of a combination of these two genera used to predict lung cancer was 0.888 (sensitivity = 95.0%, specificity = 75.0% and sensitivity 70.0%, specificity = 100.0%; p = 0.002). Conclusion: The results indicate that differences exist in the bacterial communities of patients with lung cancer and those with benign mass-like lesions. The genera Veillonella and Megasphaera showed the potential to serve as biomarkers to predict lung cancer. Thus, the lung microbiota may change the environment in patients with lung cancer. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
ISSN
0169-5002
URI
http://hdl.handle.net/10371/165739
DOI
https://doi.org/10.1016/j.lungcan.2016.10.016
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College of Natural Sciences (자연과학대학)Dept. of Biological Sciences (생명과학부)Journal Papers (저널논문_생명과학부)
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