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Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers

Cited 9 time in Web of Science Cited 9 time in Scopus
Authors

Kim, Kwanghee; An, Hyo Jin; Jun, Seung-Hyun; Kim, Tae-Jin; Lim, Seon Ah; Park, Gayoung; Na, Hyon Bin; Park, Yong Il; Hyeon, Taeghwan; Yee, Cassian; Bluestone, Jeffrey A.; Kim, Jungbae; Lee, Kyung-Mi

Issue Date
2012-08
Publisher
American Chemical Society
Citation
Nano Letters, Vol.12 No.8, pp.4018-4024
Abstract
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3(+) T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8(+)CD3(+) T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
ISSN
1530-6984
URI
https://hdl.handle.net/10371/166207
DOI
https://doi.org/10.1021/nl301388d
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  • College of Engineering
  • School of Chemical and Biological Engineering
Research Area Chemistry, Materials Science

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