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Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts

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Authors
Lee, Jeong Hyo; Kim, Seo Woo; Han, Ji Seon; Shin, Seung Pyo; Lee, Sang In; Park, Tae Sub
Issue Date
2020-05-29
Publisher
BMC
Citation
BMC Molecular and Cell Biology. 2020 May 29;21(1):40
Abstract
Background
In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, and more efficient animal production. Chicken myoblast cells, which are required for muscle development and regeneration, are particularly important because chicken growth is closely related to muscle mass.

Results
In this study, we induced expression of microRNA-146b-5p mediated by the piggyBac transposon system in primary chicken myoblast (pCM) cells. Subsequently, we analyzed and compared the proliferation and differentiation capacity and also examined the expression of related genes in regular pCM (rpCM) cells and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells). pCM-146b OE cells showed increased proliferation and upregulated gene expression related to cell proliferation. In addition, next-generation sequencing analyses were performed to compare global gene expression patterns between rpCM cells and pCM-146b OE cells. We found that the higher proliferation in pCM-146b OE cells was the result of upregulation of gene sets related to the cell cycle. Moreover, miRNA-146b-5p overexpression had inhibitory effects on myotube differentiation in pCM cells.

Conclusions
Collectively these results demonstrate that miR-146b-5p is closely related to the proliferation and differentiation of chicken myogenic cells as a modulator of post-transcription.
ISSN
2661-8850
Language
English
URI
http://hdl.handle.net/10371/168584
DOI
https://doi.org/10.1186/s12860-020-00284-z
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Journal Papers (저널논문_국제농업기술학과)
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