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Control of Membrane Biofouling in MBR for Wastewater Treatment by Quorum Quenching Bacteria, Pseudomonas sp. 1A1 : 하폐수 처리용 분리막 생물반응기에서 정족수 감지 억제 미생물에 의한 생물막 오염 제어
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이정학 | - |
dc.contributor.author | 문윤희 | - |
dc.date.accessioned | 2020-12-28T11:53:35Z | - |
dc.date.available | 2020-12-28T11:53:35Z | - |
dc.date.issued | 2012 | - |
dc.identifier.other | 000000003700 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171413 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000003700 | ko_KR |
dc.description.abstract | Recently, interspecies quorum quenching by bacterial cell has been elaborated and proved to be more efficient and economic than enzymatic quorum quenching for the biofouling control in membrane bioreactor (MBR) for wastewater treatment. However, since the activated sludge of MBRs have diverse characteristics according to the source of wastewater, it is necessary to secure diverse species of quorum quenching bacteria to apply them widely and efficiently to MBRs with diverse feed sources. The purpose of this study is to isolate and characterize quorum quenching bacteria from a lab-scale MBR and to find optimum environment for each quorum quenching bacterium. A quorum quenching Pseudomonas sp. 1A1 was chosen among isolates as a representative bacterium which produces a quorum quenching enzyme extracellularly. The AHL degradation efficiency of Pseudomonas sp.1A1 was compared with that of Rhodococcus sp. BH4 which has been reported to produce a quorum quenching enzyme intracellularly. Both bacteria were encapsulated in microporous MF (pore size, 0.4 μm) and UF(pore size, 0.03μm) hollow fiber membranes, respectively. The microbial encapsulated vessel was submerged into a continuous MBR together with a filtration membrane to test its efficiency in the biofouling inhibition. By monitoring the change in transmembrane pressure (TMP), the Pseudomonas sp. 1A1 producing an extracellular quorum quenching enzyme was confirmed to be as efficient in biofouling control as the Rhodococcus sp. BH4 producing an intracellular quorum quenching enzyme. However, the quorum quenching efficiency of Pseudomonas sp 1A1 was different dependent on the porosity of vessel (i.e., MF or UF hollow fiber) in which this bacterium was entrapped. It could be explained by the mass transfer of signal molecules and enzymes through the pores of vessel. | - |
dc.description.tableofcontents | Abstracts
Contents List of Figures List of Tables I Introduction I.1 Background I.2 Objective II Literature Review II.1 Membrane bioreactor (MBR) in advanced wastewater treatment II.1.1 Membrane bioreactor (MBR) system II.1.2 Development of membrane bioreactor (MBR) process II.1.3 Advantages and challenges of MBR process II.2 Membrane fouling in MBR II.2.1 Membrane fouling mechanism II.2.2 Classification of membrane fouling II.2.3 Factors affecting membrane fouling II.2.4 Membrane fouling control II.3 Biofilm on the membrane surface II.3.1 Basic of biofilm II.3.2 Biofilm in the MBR II.4 Quorum Sensing II.4.1 QS in Gram-negative bacteria II.4.2 QS in Gram-positive bacteria II.4.3 Autoinducers-2 (AI2) for the interspecies communication II.4.4 The connections between QS and biofilms II.4.5 QS control strategies II.5 Quorum quenching (QQ) II.5.1 Enzymatic quorum quenching II.5.2 Bacterial quorum quenching III Materials and Methods III.1 Isolation of AHL degrading bacteria III.1.1 Bacterial strains, media, and culture condition III.1.2 Enrichment and isolation procedures III.2 Detection of AHL molecules III.2.1 Reporter strain for the AHLs detection III.2.2 Cross feeding bioassay III.2.3 Quorum quenching activity III.3 Entrapment of quorum quenching bacteria III.4 MBR operation condition IV Results and discussion IV.1 Isolation of quorum quenching bacteria IV.1.1 Identification of isolates IV.1.2 Selection of quorum quenching bacteria not producing AHL IV.1.3 Comparison of AHL degrading activity between isolated bacteria IV.2 Comparison of N-acyl homoserin lacton (AHL) degrading activity between two bacteria; Pseudomonas sp. and Rhodococcus sp. IV.2.1 Characterization of quorum quenching enzymes produced by the two bacteria IV.2.2 AHL degrading activity of quorum quenching bacteria encapsulated in Microbial-Vessel with different pore sizes IV.3 Application of Pseudomonas sp. and Rhodococcus sp. in continuous MBR system IV.3.1 Inhibition of membrane biofouling in MBR using microfilter (MF) membrane vessel IV.3.2 Inhibition of membrane biofouling in MBR using ultrafilter (UF) membrane vessel V Conclusion Reference | - |
dc.format.extent | ⅸ,95 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Membrane bioreactor (MBR) | - |
dc.subject | Quorum sensing | - |
dc.subject | Quorum quenching bacteria | - |
dc.subject.ddc | 660.6 | - |
dc.title | Control of Membrane Biofouling in MBR for Wastewater Treatment by Quorum Quenching Bacteria, Pseudomonas sp. 1A1 | - |
dc.title.alternative | 하폐수 처리용 분리막 생물반응기에서 정족수 감지 억제 미생물에 의한 생물막 오염 제어 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.department | 공과대학 화학생물공학부 | - |
dc.description.degree | Master | - |
dc.date.awarded | 2012-08 | - |
dc.identifier.holdings | 000000000012▲000000000014▲000000003700▲ | - |
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