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Extracellular vesicle-associated miR-135b and -135a regulate stemness in Group 4 medulloblastoma cells by targeting angiomotin-like 2
DC Field | Value | Language |
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dc.contributor.author | Choi, Seung A | - |
dc.contributor.author | Koh, Eun J | - |
dc.contributor.author | Kim, Ryong N | - |
dc.contributor.author | Byun, Jung W | - |
dc.contributor.author | Phi, Ji H | - |
dc.contributor.author | Yang, Jeyul | - |
dc.contributor.author | Wang, Kyu-Chang | - |
dc.contributor.author | Park, Ae K | - |
dc.contributor.author | Hwang, Do W | - |
dc.contributor.author | Lee, Ji Y | - |
dc.contributor.author | Kim, Seung-Ki | - |
dc.date.accessioned | 2021-01-27T05:45:09Z | - |
dc.date.available | 2021-01-27T05:45:09Z | - |
dc.date.issued | 2020-11-20 | - |
dc.identifier.citation | Cancer Cell International. 2020 Nov 20;20(1):558 | - |
dc.identifier.uri | https://doi.org/10.1186/s12935-020-01645-6 | - |
dc.identifier.uri | https://hdl.handle.net/10371/171712 | - |
dc.description.abstract | Abstract
Background Extracellular vesicles (EVs) secreted by tumours, including exosomes, are important factors that regulate cell–cell interactions in oncogenesis. Although EV studies are ongoing, the biological understanding of EV-miRNAs derived from brain tumour spheroid-forming cells (BTSCs) of medulloblastoma is poor. Purposes We explored the specific cellular miRNAs and EV-miRNAs in medulloblastoma BTSCs to determine their potential biological function. Methods Bulk tumor cells (BTCs) and BTSCs were cultured under different conditions from medulloblastoma tissues (N = 10). Results Twenty-four miRNAs were simultaneously increased in both cells and EVs derived from BTSCs in comparison to BTCs. After inhibition of miR-135b or miR135a which were the most significantly increased in BTSCs, cell viability, self-renewal and stem cell marker expression decreased remarkably. Through integrated analysis of mRNAs and miRNAs data, we found that angiomotin-like 2 (AMOTL2), which was significantly decreased, was targeted by both miR-135b and miR-135a. STAT6 and GPX8 were targeted only by miR-135a. Importantly, low expression of AMOTL2 was significantly associated with overall poor survival in paediatric Group 3 and Group 4 medulloblastoma patients. Conclusion Our results indicated that inhibition of miR-135b or miR-135a leads to suppress stemness of BTSC through modulation of AMOTL2. | - |
dc.title | Extracellular vesicle-associated miR-135b and -135a regulate stemness in Group 4 medulloblastoma cells by targeting angiomotin-like 2 | - |
dc.type | Journal Article | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2020-11-22T04:31:29Z | - |
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