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Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice
DC Field | Value | Language |
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dc.contributor.author | Minai-Tehrani, Arash | - |
dc.contributor.author | Park, Young-Chan | - |
dc.contributor.author | Hwang, Soon-Kyung | - |
dc.contributor.author | Kwon, Jung-Taek | - |
dc.contributor.author | Chang, Seung-Hee | - |
dc.contributor.author | Park, Sung-Jin | - |
dc.contributor.author | Yu, Kyeong-Nam | - |
dc.contributor.author | Kim, Ji-Eun | - |
dc.contributor.author | Shin, Ji-Young | - |
dc.contributor.author | Kim, Ji-Hye | - |
dc.contributor.author | Kang, Bitna | - |
dc.contributor.author | Hong, Seong-Ho | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.date.accessioned | 2021-01-31T08:47:33Z | - |
dc.date.available | 2021-01-31T08:47:33Z | - |
dc.date.created | 2020-12-15 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | Journal of Veterinary Science, Vol.12 No.4, pp.309-317 | - |
dc.identifier.issn | 1229-845X | - |
dc.identifier.other | 119159 | - |
dc.identifier.uri | https://hdl.handle.net/10371/172461 | - |
dc.description.abstract | Conventional lung cancer therapies are associated with poor survival rates; therefore, new approaches such as gene therapy are required for treating cancer. Gene therapies for treating lung cancer patients can involve several approaches. Among these, aerosol gene delivery is a potentially more effective approach. In this study, Akt1 kinase-deficient (KD) and wild-type (WT) Akt1 were delivered to the lungs of CMV-LucR-cMyc-IRES-LucF dual reporter mice through a nose only inhalation system using glucosylated polyethylenimine and naphthalene was administrated to the mice via intraperitoneal injection. Aerosol delivery of Akt1 WT and naphthalene treatment increased protein levels of downstream substrates of Akt signaling pathway while aerosol delivery of Akt1 KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Aka KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after delivering Aka KD. These studies demonstrated that our aerosol delivery is compatible for in vivo gene delivery. | - |
dc.language | 영어 | - |
dc.publisher | 대한수의학회 | - |
dc.title | Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.4142/jvs.2011.12.4.309 | - |
dc.citation.journaltitle | Journal of Veterinary Science | - |
dc.identifier.wosid | 000298453500002 | - |
dc.identifier.scopusid | 2-s2.0-84860748232 | - |
dc.citation.endpage | 317 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 309 | - |
dc.citation.volume | 12 | - |
dc.identifier.sci | 000298453500002 | - |
dc.identifier.kciid | ART001612960 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE/AKT PATHWAY | - |
dc.subject.keywordPlus | P53 GENE-TRANSFER | - |
dc.subject.keywordPlus | GLUCOSYLATED POLYETHYLENIMINE | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | REPORTER MICE | - |
dc.subject.keywordPlus | C-MYC | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | INITIATION | - |
dc.subject.keywordAuthor | aerosol delivery | - |
dc.subject.keywordAuthor | Akt1 | - |
dc.subject.keywordAuthor | Clara cell | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | luciferase activity | - |
dc.subject.keywordAuthor | naphthalene | - |
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