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Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Soo-Ji | - |
dc.contributor.author | Jeong, Tae Yeong | - |
dc.contributor.author | Shin, Seung Kyun | - |
dc.contributor.author | Yoon, Da Eun | - |
dc.contributor.author | Lim, Soo-Yeon | - |
dc.contributor.author | Kim, Sol Pin | - |
dc.contributor.author | Choi, Jungmin | - |
dc.contributor.author | Lee, Hyunji | - |
dc.contributor.author | Hong, Jeong-Im | - |
dc.contributor.author | Ahn, Jinhee | - |
dc.contributor.author | Seong, Je Kyung | - |
dc.contributor.author | Kim, Kyoungmi | - |
dc.date.accessioned | 2021-08-12T01:01:03Z | - |
dc.date.available | 2021-08-12T10:16:47Z | - |
dc.date.issued | 2021-06-03 | - |
dc.identifier.citation | Genome Biology. 2021 Jun 03;22(1):170 | ko_KR |
dc.identifier.issn | 1474-760X | - |
dc.identifier.uri | https://hdl.handle.net/10371/174784 | - |
dc.description.abstract | Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to improve the editing efficiency using proximal dead sgRNA and chromatin-modulating peptides. We used enhanced prime-editing to generate Igf2 mutant mice with editing frequencies of up to 47% and observed germline transmission, no off-target effects, and a dwarf phenotype. This improved prime-editing method can be efficiently applied to cell research and to generate mouse models. | ko_KR |
dc.description.sponsorship | This study was supported by the Chung Yang, Cha Young Sun, & Jang Hi Joo Memorial Fund. This study was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) of Korea (Korea Mouse Phenotyping Project, NRF-2013M3A9D5072550, NRF-2020M3A9D5A01082439, NRF2019R1A2C2087198, and NRF- 2019M3A9H1103792). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Prime editor | - |
dc.subject | Igf2 | - |
dc.subject | Adamts20 | - |
dc.subject | Mouse cells and embryos | - |
dc.subject | Germline transmission | - |
dc.subject | Dwarf phenotype | - |
dc.subject | Proximal dead sgRNA | - |
dc.subject | Chromatin-modulating peptides | - |
dc.title | Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박수지 | - |
dc.contributor.AlternativeAuthor | 정태영 | - |
dc.contributor.AlternativeAuthor | 신승균 | - |
dc.contributor.AlternativeAuthor | 윤다은 | - |
dc.contributor.AlternativeAuthor | 임수연 | - |
dc.contributor.AlternativeAuthor | 김솔빈 | - |
dc.contributor.AlternativeAuthor | 최정민 | - |
dc.contributor.AlternativeAuthor | 이현지 | - |
dc.contributor.AlternativeAuthor | 홍정임 | - |
dc.contributor.AlternativeAuthor | 안지희 | - |
dc.contributor.AlternativeAuthor | 성제경 | - |
dc.contributor.AlternativeAuthor | 김경미 | - |
dc.identifier.doi | 10.1186/s13059-021-02389-w | - |
dc.citation.journaltitle | Genome Biology | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2021-06-06T03:14:15Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 170 | ko_KR |
dc.citation.volume | 22 | ko_KR |
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