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A Lipid-Nanopillar-Array-Based Immunosorbent Assay for Virus Detection : 지질이중층 나노필라어레이기반 면역 흡착법을 통한 바이러스 검지
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- Authors
- Advisor
- 남좌민
- Issue Date
- 2021-02
- Publisher
- 서울대학교 대학원
- Keywords
- biosensors ; immunosorbent assays ; lipid nanopillar arrays ; supported lipid bilayers ; virus detection ; 바이오센서 ; 면역흡착 어세이 ; 지질 나노필라 어레이 ; 지지형 지질 이중층 ; 바이러스 검지
- Description
- 학위논문 (박사) -- 서울대학교 대학원 : 자연과학대학 화학과, 2021. 2. 남좌민.
- Abstract
- Since infectious diseases, particularly viral infections, have threatened human health and caused huge economical losses globally, a rapid, sensitive, and selective virus detection platform is highly demanded. Enzyme‐linked immunosorbent assay (ELISA) with flat solid substrates has been dominantly used in detecting whole viruses for its straightforwardness and simplicity in assay protocols, but it often suffers from limited sensitivity, poor quantification range, and a time‐consuming assay procedure.
Here, a lipid‐nanopillar‐array‐based immunosorbent assay (LNAIA) is developed with a nanopillar‐supported lipid bilayer substrate with fluorophore‐modified antibodies for rapid, sensitive, and quantitative detection of viruses. 3D- nanopillar array structures and fluid antibodies with fluorophores facilitate faster and efficient target binding and rapid fluorophore localization for quick, reliable analysis on binding events with a conventional fluorescence microscopy setup. LNAIA enables quantification of the H1N1 virus that targets down to 150 virus particles with the 5‐orders‐of‐magnitude dynamic range within 25 min, which cannot be achieved with conventional ELISA platforms.
- Language
- eng
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