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Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Annie | - |
dc.contributor.author | Lee, Kun-Gu | - |
dc.contributor.author | Kwon, Yeongbeen | - |
dc.contributor.author | Lee, Kang-In | - |
dc.contributor.author | Yang, Heung-Mo | - |
dc.contributor.author | Habib, Omer | - |
dc.contributor.author | Kim, Jihun | - |
dc.contributor.author | Kim, Sang-Tae | - |
dc.contributor.author | Kim, Sung Joo | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.contributor.author | Hwang, Dong-Youn | - |
dc.date.accessioned | 2022-04-20T10:55:34Z | - |
dc.date.available | 2022-04-20T10:55:34Z | - |
dc.date.created | 2021-07-06 | - |
dc.date.created | 2021-07-06 | - |
dc.date.created | 2021-07-06 | - |
dc.date.created | 2021-07-06 | - |
dc.date.issued | 2021-06 | - |
dc.identifier.citation | Stem Cell Reviews and Reports, Vol.17 No.3, pp.1053-1067 | - |
dc.identifier.issn | 2629-3269 | - |
dc.identifier.other | 136994 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179109 | - |
dc.description.abstract | Human embryonic stem cells (hESCs) hold promise in regenerative medicine but allogeneic immune rejections caused by highly polymorphic human leukocyte antigens (HLAs) remain a barrier to their clinical applications. Here, we used a CRISPR/Cas9-mediated HLA-editing strategy to generate a variety of HLA homozygous-like hESC lines from pre-established hESC lines. We edited four pre-established HLA-heterozygous hESC lines and created a mini library of 14 HLA-edited hESC lines in which single HLA-A and HLA-B alleles and both HLA-DR alleles are disrupted. The HLA-edited hESC derivatives elicited both low T cell- and low NK cell-mediated immune responses. Our library would cover about 40% of the Asian-Pacific population. We estimate that HLA-editing of only 19 pre-established hESC lines would give rise to 46 different hESC lines to cover 90% of the Asian-Pacific population. This study offers an opportunity to generate an off-the-shelf HLA-compatible hESC bank, available for immune-compatible cell transplantation, without embryo destruction. Graphical | - |
dc.language | 영어 | - |
dc.publisher | HUMANA PRESS INC | - |
dc.title | Off-the-Shelf, Immune-Compatible Human Embryonic Stem Cells Generated Via CRISPR-Mediated Genome Editing | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 김진수 | - |
dc.identifier.doi | 10.1007/s12015-020-10113-7 | - |
dc.citation.journaltitle | Stem Cell Reviews and Reports | - |
dc.identifier.wosid | 000606409400001 | - |
dc.identifier.scopusid | 2-s2.0-85098973871 | - |
dc.citation.endpage | 1067 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 1053 | - |
dc.citation.volume | 17 | - |
dc.identifier.sci | 000606409400001 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Soo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MESSENGER-RNA DECAY | - |
dc.subject.keywordPlus | CLASS-I | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | HLA | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | REJECTION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | FREQUENCIES | - |
dc.subject.keywordAuthor | Human embryonic stem cells | - |
dc.subject.keywordAuthor | HLA-editing | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Cas9 | - |
dc.subject.keywordAuthor | HLA homozygous-like hESCs | - |
dc.subject.keywordAuthor | Immune-compatible hESC banking | - |
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