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A chemical tool for blue light-inducible proximity photo-crosslinking in live cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mishra, Pratyush Kumar | - |
dc.contributor.author | Kang, Myeong-Gyun | - |
dc.contributor.author | Lee, Hakbong | - |
dc.contributor.author | Kim, Seungjoon | - |
dc.contributor.author | Choi, Subin | - |
dc.contributor.author | Sharma, Nirmali | - |
dc.contributor.author | Park, Cheol-Min | - |
dc.contributor.author | Ko, Jaewon | - |
dc.contributor.author | Lee, Changwook | - |
dc.contributor.author | Seo, Jeong Kon | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.date.accessioned | 2022-05-04T01:45:41Z | - |
dc.date.available | 2022-05-04T01:45:41Z | - |
dc.date.created | 2022-01-03 | - |
dc.date.created | 2022-01-03 | - |
dc.date.created | 2022-01-03 | - |
dc.date.issued | 2022-01-28 | - |
dc.identifier.citation | Chemical Science, Vol.13 No.4, pp.955-966 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179377 | - |
dc.description.abstract | We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient bluelight-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photocrosslinked products of various HaloTag-conjugated proteins of interest were detected in subcellular spaces in live cells. Chemical or heat stress-induced dynamic changes in the proteome were also detected, and photo-crosslinking in the mouse brain tissue was enabled. Using Spotlight, we further identified the host interactome of SARS-CoV-2 nucleocapsid (N) protein, which is essential for viral genome assembly. Mass analysis of the VL1-crosslinked product of N-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells. | - |
dc.language | 영어 | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | A chemical tool for blue light-inducible proximity photo-crosslinking in live cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1039/d1sc04871f | - |
dc.citation.journaltitle | Chemical Science | - |
dc.identifier.wosid | 000732863800001 | - |
dc.identifier.scopusid | 2-s2.0-85123741409 | - |
dc.citation.endpage | 966 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 955 | - |
dc.citation.volume | 13 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Kon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROTEIN-PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | HYDROGEN-SULFIDE | - |
dc.subject.keywordPlus | ARYL AZIDE | - |
dc.subject.keywordPlus | TAG | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | HALOTAG7 | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | STAUFEN | - |
dc.subject.keywordPlus | LIGASE | - |
dc.subject.keywordPlus | PROBES | - |
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