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Injection laryngoplasty of human adipose-derived stem cell spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynx
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Doh Young Lee | - |
dc.contributor.author | Young Hwan Choi | - |
dc.contributor.author | Ji Suk Choi | - |
dc.contributor.author | Min Rye Eom | - |
dc.contributor.author | Seong Keun Kwon | - |
dc.date.accessioned | 2022-05-10T02:17:02Z | - |
dc.date.available | 2022-05-10T02:17:02Z | - |
dc.date.issued | 2022-04-05 | - |
dc.identifier.citation | Biomaterials Research. Vol 26(1):13 | ko_KR |
dc.identifier.issn | 2055-7124 | - |
dc.identifier.uri | https://hdl.handle.net/10371/179649 | - |
dc.description.abstract | As the geriatric population increased, the need of treatment for laryngeal atrophy and dysfunction increased. This study was performed to evaluate the effects of injection of human adipose-derived stem cell (hASC) spheroid-loaded catechol-conjugated hyaluronic acid (HA-CA) hydrogel on therapeutic rejuvenation of the geriatric larynx.
Stem cell spheroids with hyaluronic acid-based hydrogel were injected into the laryngeal muscles of 18-month-old Sprague–Dawley rats. The effects of hASC spheroids were examined in the following four groups: SHAM, injected with PBS; GEL, injected with HA-CA hydrogel; MONO, injected with single hASCs in HA-CA hydrogel; and SP, injected with hASCs spheroids in HA-CA hydrogel. The rejuvenation efficacy in geriatric laryngeal muscle tissues at 12 weeks postinjection was evaluated and compared by histology, immunofluorescence staining, and functionality analysis. Total myofiber cross-sectional area and myofiber number/density, evaluated by detection of myosin heavy chain with antibodies against laminin and fast myosin heavy chain, were significantly higher in the SP group than in the other groups. The lamina propria of the larynx was evaluated by alcian blue staining, which showed that the HA was increased significantly in the SP group compared to the other groups. In functional analysis, the glottal gap area was significantly reduced in the SP group compared to the other groups. The phase difference in the vocal fold during vibration was also smaller in the SP group than in the other groups, but the difference did not reach statistical significance. Injection of hASC spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynx. | ko_KR |
dc.description.sponsorship | This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT), Republic of Korea (No. 2019M3A9H1103617 and No. 2020R1A4A4079931), a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded
by the Ministry of Health & Welfare, Republic of Korea (No. HI14C1277) and the Tech-nology Innovation Program (or Industrial Strategic Technology Development Program-Bio-industrial technology development- customized diagnostic treatment products) (No. 20014955, Devel-opment of Intelligent Automation System for mass production of cell therapy products) funded By the Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Geriatric larynx | - |
dc.subject | Stem cell | - |
dc.subject | Spheroids | - |
dc.subject | Presbylaryngis | - |
dc.subject | Injection laryngoplasty | - |
dc.title | Injection laryngoplasty of human adipose-derived stem cell spheroids with hyaluronic acid-based hydrogel improves the morphological and functional characteristics of geriatric larynx | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | https://doi.org/10.1186/s40824-022-00261-x | ko_KR |
dc.citation.journaltitle | Biomaterials Research | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-04-10T03:18:17Z | - |
dc.citation.startpage | 13 | ko_KR |
dc.citation.volume | 26 | ko_KR |
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