N-6-methyladenosine-modified RNA acts as a molecular glue that drives liquid-liquid phase separation in plants

Abstract

Liquid-like condensates are organized by multivalent intrinsically disordered proteins and RNA molecules. We here demonstrate that N-6-methyladenosine (m(6)A)-modified RNA is widespread in establishing diverse plant cell condensates. Several m(6)A-reader proteins contain putative prion-like domains, and the ect2/3/4 mutant exhibited reduced formation of key nuclear and cytoplasmic condensates in Arabidopsis.