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Modulation of osteogenic differentiation by Escherichia coli-derived recombinant bone morphogenetic protein-2
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Nam-Hyun | - |
dc.contributor.author | Jung, Seon-Kyong | - |
dc.contributor.author | Lee, Juno | - |
dc.contributor.author | Chang, Pahn-Shick | - |
dc.contributor.author | Kang, Seung-Hoon | - |
dc.date.accessioned | 2022-10-04T08:28:32Z | - |
dc.date.available | 2022-10-04T08:28:32Z | - |
dc.date.created | 2022-08-23 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.citation | AMB Express, Vol.12 No.1, p. 106 | - |
dc.identifier.issn | 2191-0855 | - |
dc.identifier.uri | https://hdl.handle.net/10371/185250 | - |
dc.description.abstract | Recombinant human bone morphogenetic protein-2 (rhBMP-2), a key regulator of osteogenesis, induces the differentiation of mesenchymal cells into cartilage or bone tissues. Early orthopedic and dental studies often used mammalian cell-derived rhBMP-2, especially Chinese hamster ovary (CHO) cells. However, CHO cell-derived rhBMP-2 (C-rhBMP-2) presents disadvantages such as high cost and low production yield. To overcome these problems, Escherichia coli-derived BMP-2 (E-rhBMP-2) was developed; however, the E-rhBMP-2-induced signaling pathways and gene expression profiles during osteogenesis remain unclear. Here, we investigated the E-rhBMP-2-induced osteogenic differentiation pattern in C2C12 cells and elucidated the difference in biological characteristics between E-rhBMP-2 and C-rhBMP-2 via surface plasmon resonance, western blotting, qRT-PCR, RNA-seq, and alkaline phosphatase assays. The binding affinities of E-rhBMP-2 and C-rhBMP-2 towards BMP receptors were similar, both being confirmed at the nanomolecular level. However, the phosphorylation of Smad1/5/9 at 3 h after treatment with E-rhBMP-2 was significantly lower than that on treatment with C-rhBMP-2. The expression profiles of osteogenic marker genes were similar in both the E-rhBMP-2 and C-rhBMP-2 groups, but the gene expression level in the E-rhBMP-2 group was lower than that in the C-rhBMP-2 group at each time point. Taken together, our results suggest that the osteogenic signaling pathways induced by E-rhBMP-2 and C-rhBMP-2 both follow the general Smad-signaling pathway, but the difference in intracellular phosphorylation intensity results in distinguishable transcription profiles on osteogenic marker genes and biological activities of each rhBMP-2. These findings provide an extensive understanding of the biological properties of E-rhBMP-2 and the signaling pathways during osteogenic differentiation. | - |
dc.language | 영어 | - |
dc.publisher | Springer Verlag | - |
dc.title | Modulation of osteogenic differentiation by Escherichia coli-derived recombinant bone morphogenetic protein-2 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13568-022-01443-5 | - |
dc.citation.journaltitle | AMB Express | - |
dc.identifier.wosid | 000838644400002 | - |
dc.identifier.scopusid | 2-s2.0-85135623078 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 106 | - |
dc.citation.volume | 12 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Chang, Pahn-Shick | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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