Publications
Detailed Information
A high-throughput biomimetic bone-on-a-chip platform with artificial intelligence-assisted image analysis for osteoporosis drug testing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Paek, Kyurim | - |
dc.contributor.author | Kim, Seulha | - |
dc.contributor.author | Tak, Sungho | - |
dc.contributor.author | Kim, Min Kyeong | - |
dc.contributor.author | Park, Jubin | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.contributor.author | Kim, Jeong Ah | - |
dc.date.accessioned | 2022-10-07T01:27:32Z | - |
dc.date.available | 2022-10-07T01:27:32Z | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.created | 2022-09-13 | - |
dc.date.issued | 2023-01 | - |
dc.identifier.citation | Bioengineering & Translational Medicine, Vol.8 No.1 | - |
dc.identifier.issn | 2380-6761 | - |
dc.identifier.uri | https://hdl.handle.net/10371/185574 | - |
dc.description.abstract | Although numerous organ-on-a-chips have been developed, bone-on-a-chip platforms have rarely been reported because of the high complexity of the bone microenvironment. With an increase in the elderly population, a high-risk group for bone-related diseases such as osteoporosis, it is essential to develop a precise bone-mimicking model for efficient drug screening and accurate evaluation in preclinical studies. Here, we developed a high-throughput biomimetic bone-on-a-chip platform combined with an artificial intelligence (AI)-based image analysis system. To recapitulate the key aspects of natural bone microenvironment, mouse osteocytes (IDG-SW3) and osteoblasts (MC3T3-E1) were cocultured within the osteoblast-derived decellularized extracellular matrix (OB-dECM) built in a well plate-based three-dimensional gel unit. This platform spatiotemporally and configurationally mimics the characteristics of the structural bone unit, known as the osteon. Combinations of native and bioactive ingredients obtained from the OB-dECM and coculture of two types of bone cells synergistically enhanced osteogenic functions such as osteocyte differentiation and osteoblast maturation. This platform provides a uniform and transparent imaging window that facilitates the observation of cell-cell interactions and features high-throughput bone units in a well plate that is compatible with a high-content screening system, enabling fast and easy drug tests. The drug efficacy of anti-SOST antibody, which is a newly developed osteoporosis drug for bone formation, was tested via beta-catenin translocation analysis, and the performance of the platform was evaluated using AI-based deep learning analysis. This platform could be a cutting-edge translational tool for bone-related diseases and an efficient alternative to bone models for the development of promising drugs. | - |
dc.language | 영어 | - |
dc.publisher | Wiley | American Institute of Chemical Engineers; Society for Biological Engineering | - |
dc.title | A high-throughput biomimetic bone-on-a-chip platform with artificial intelligence-assisted image analysis for osteoporosis drug testing | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/btm2.10313 | - |
dc.citation.journaltitle | Bioengineering & Translational Medicine | - |
dc.identifier.wosid | 000777969500001 | - |
dc.identifier.scopusid | 2-s2.0-85127429376 | - |
dc.citation.number | 1 | - |
dc.citation.volume | 8 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Tai Hyun | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | OSTEOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | ROMOSOZUMAB | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordAuthor | bone formation | - |
dc.subject.keywordAuthor | bone-on-a-chip | - |
dc.subject.keywordAuthor | deep learning | - |
dc.subject.keywordAuthor | drug testing | - |
dc.subject.keywordAuthor | high-throughput analysis | - |
dc.subject.keywordAuthor | osteoporosis | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.