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Callerya atropurpurea suppresses inflammation in vitro and ameliorates gastric injury as well as septic shock in vivo via TLR4/MyD88-dependent cascade
DC Field | Value | Language |
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dc.contributor.author | You, Long | - |
dc.contributor.author | Huang, Lei | - |
dc.contributor.author | Jang, Jiwon | - |
dc.contributor.author | Hong, Yo Han | - |
dc.contributor.author | Kim, Han Gyung | - |
dc.contributor.author | Chen, Hongxi | - |
dc.contributor.author | Shin, Chae Yun | - |
dc.contributor.author | Yoon, Ji Hye | - |
dc.contributor.author | Manilack, Philaxay | - |
dc.contributor.author | Sounyvong, Bounthan | - |
dc.contributor.author | Lee, Woo-Shin | - |
dc.contributor.author | Jeon, Mi-Jeong | - |
dc.contributor.author | Lee, Sarah | - |
dc.contributor.author | Lee, Byoung-Hee | - |
dc.contributor.author | Cho, Jae Youl | - |
dc.date.accessioned | 2022-10-11T00:27:52Z | - |
dc.date.available | 2022-10-11T00:27:52Z | - |
dc.date.created | 2022-09-29 | - |
dc.date.issued | 2022-10 | - |
dc.identifier.citation | Phytomedicine, Vol.105, p. 154338 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.uri | https://hdl.handle.net/10371/185651 | - |
dc.description.abstract | © 2022Background: Callerya atropurpurea is a traditional plant in a tropical zone discovered to have anti-inflammatory functions. Purpose: we want to investigate the mechanism related to anti-inflammation of C. atropurpurea ethanol extract (Ca-EE) both in vitro and in vivo. Study design: Murine macrophage cells and mouse models for gastritis and septic shock were conducted to evaluate the abilities of Ca-EE in anti-inflammation. Methods: Ca-EE was tested by HPLC and LC-MS/MS. NO outcome was checked by Griess reagent test. Cell viabilities were evaluated using MTT assay. Inflammatory cytokines were determined via RT-PCR and ELISA. The mechanism of Ca-EE in anti-inflammation was investigated by luciferase reporter gene assay and immunoblot in transcription level and protein level respectively. Gastric injury and septic shock administrated with Ca-EE were studied by H&E, PCR, and immunoblot. Results: Ca-EE significantly decreased LPS-induced NO production, but hardly stimulated the expression of NO itself. It not only showed no cytotoxicity, but also protected cells from LPS damage. Moreover, Ca-EE decreased TLR4 expression, altered MyD88 recruitment and TRAF6, and suppressed the phospho-Src/PI3K/AKT. Ca-EE inhibited downstream signaling P38, JNK and NF-κB. Finally, Ca-EE alleviated HCl/EtOH-induced gastritis and LPS/poly (I:C)-induced septic shock through the previously mentioned signaling cascades. Conclusion: Ca-EE exhibited an integrated and promising mechanism against TLR4-related inflammation, which shows potential for treating gastritis, septic shock, and other inflammatory diseases. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier GmbH | - |
dc.title | Callerya atropurpurea suppresses inflammation in vitro and ameliorates gastric injury as well as septic shock in vivo via TLR4/MyD88-dependent cascade | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.phymed.2022.154338 | - |
dc.citation.journaltitle | Phytomedicine | - |
dc.identifier.scopusid | 2-s2.0-85135507548 | - |
dc.citation.startpage | 154338 | - |
dc.citation.volume | 105 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Woo-Shin | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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