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Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seonghyun | - |
dc.contributor.author | Lee, Hyunji | - |
dc.contributor.author | Baek, Gayoung | - |
dc.contributor.author | Namgung, Eunji | - |
dc.contributor.author | Park, Joo M. | - |
dc.contributor.author | Kim, Sanghun | - |
dc.contributor.author | Hong, Seongho | - |
dc.contributor.author | Kim, Jin-Soo | - |
dc.date.accessioned | 2022-10-17T05:16:44Z | - |
dc.date.available | 2022-10-17T05:16:44Z | - |
dc.date.issued | 2022-10-12 | - |
dc.identifier.citation | Genome Biology. 2022 Oct 12;23(1):211 | - |
dc.identifier.uri | https://doi.org/10.1186/s13059-022-02782-z | - |
dc.identifier.uri | https://hdl.handle.net/10371/186387 | - |
dc.description.abstract | Abstract
We present two methods for enhancing the efficiency of mitochondrial DNA (mtDNA) editing in mice with DddA-derived cytosine base editors (DdCBEs). First, we fused DdCBEs to a nuclear export signal (DdCBE-NES) to avoid off-target C-to-T conversions in the nuclear genome and improve editing efficiency in mtDNA. Second, mtDNA-targeted TALENs (mitoTALENs) are co-injected into mouse embryos to cleave unedited mtDNA. We generated a mouse model with the m.G12918A mutation in the MT-ND5 gene, associated with mitochondrial genetic disorders in humans. The mutant mice show hunched appearances, damaged mitochondria in kidney and brown adipose tissues, and hippocampal atrophy, resulting in premature death. | - |
dc.title | Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases | - |
dc.type | Journal Article | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-10-16T03:12:07Z | - |
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