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Mannosylated-serum albumin nanoparticle imaging to monitor tumor-associated macrophages under anti-PD1 treatment
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- Authors
- Issue Date
- 2023-01-27
- Publisher
- BMC
- Citation
- Journal of Nanobiotechnology, 21(1):31
- Keywords
- Mannosylated-serum albumin ; Positron emission tomography ; Tumor microenvironment ; Tumor-associated macrophage ; Anti-PD1
- Abstract
- Background
Immune checkpoint inhibitors such as anti-programmed cell death protein 1 (PD1) block tumor growth
by reinvigorating the immune system; however, determining their efcacy only by the changes in tumor size may
prove inaccurate. As the immune cells including macrophages in the tumor microenvironment (TME) are associ‑
ated with the response to anti-PD1 therapy, tumor-associated macrophages (TAMs) imaging using nanoparticles can
noninvasively provide the immune enrichment status of TME. Herein, the mannosylated-serum albumin (MSA) nano‑
particle was labeled with radioactive isotope 68Ga to target the mannose receptors on macrophages for noninvasive
monitoring of the TME according to anti-PD1 therapy.
Results
B16F10-Luc and MC38-Luc tumor-bearing mice were treated with anti-PD1, and the response to anti-PD1
was determined by the tumor volume. According to the fow cytometry, the responders to anti-PD1 showed an
increased proportion of TAMs, as well as lymphocytes, and the most enriched immune cell population in the TME
was also TAMs. For noninvasive imaging of TAMs as a surrogate of immune cell augmentation in the TME via anti-PD1,
we acquired [
68Ga] Ga-MSA positron emission tomography. According to the imaging study, an increased number of
TAMs in responders at the early phase of anti-PD1 treatment was observed in both B16F10-Luc and MC38-Luc tumorbearing mice models.
Conclusion
As representative immune cells in the TME, non-invasive imaging of TAMs using MSA nanoparticles can
refect the immune cell enrichment status in the TME closely associated with the response to anti-PD1. As non-inva‑
sive imaging using MSA nanoparticles, this approach shows a potential to monitor and evaluate anti-tumor response
to immune checkpoint inhibitors.
- ISSN
- 1477-3155
- Language
- English
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