Publications
Detailed Information
Protective effects of leucine against lipopolysaccharide-induced inflammatory response in Labeo rohita fingerlings
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Giri, Sib Sankar | - |
dc.contributor.author | Sen, Shib Sankar | - |
dc.contributor.author | Jun, Jin Woo | - |
dc.contributor.author | Sukumaran, Venkatachalam | - |
dc.contributor.author | Park, Se Chang | - |
dc.date.accessioned | 2023-04-19T08:48:25Z | - |
dc.date.available | 2023-04-19T08:48:25Z | - |
dc.date.created | 2018-08-29 | - |
dc.date.created | 2018-08-29 | - |
dc.date.created | 2018-08-29 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | Fish and Shellfish Immunology, Vol.52, pp.239-247 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191181 | - |
dc.description.abstract | The present study investigated the protective effects of leucine against lipopolysaccharide (LPS)-induced inflammatory responses in Labeo rohita (rohu) in vivo and in vitro. Primary hepatocytes, isolated from the hepatopancreas, were exposed to different concentrations of LPS for 24 h to induce an inflammatory response, and the protective effects of leucine against LPS-induced inflammation were studied. Finally, we investigated the efficiency of dietary leucine supplementation in attenuating an immune challenge induced by LPS in vivo. Exposure of cells to 10-25 mu g mL(-1) of LPS for 24 h resulted in a significant production of nitric oxide and release of lactate dehydrogenase to the medium, whereas cell viability and protein content were reduced (p < 0.05). LPS exposure (10 mu g mL(-1)) increased mRNA levels of the pro inflammatory cytokines TNF-alpha, IL-1 beta and IL-8 in vitro (p < 0.05). However, pretreatment with leucine prevented the LPS-induced upregulation of TNF-alpha, IL-1 beta and IL-8 mRNAs by downregulating TLR4, MyD88, NF-kappa Bp65, and MAPKp38 mRNA expression. Interestingly, mRNA expression of the anti-inflammatory cytokine, IL-10, which was increased by LPS treatment, was further enhanced (p < 0.05) by leucine pretreatment. The enhanced expression of IL-10 might inhibit the production of other pro inflammatory cytokines. It was found that leucine pretreatment attenuated the excessive activation of LPS-induced TLR4-MyD88 signaling as manifested by lower level of TLR4, MyD88, MAPKp38, NF-kappa Bp65 and increased level of I kappa B-alpha protein in leucine pre-treatment group. In vivo experiments demonstrated that leucine pre-supplementation could protect fish against LPS-induced inflammation through an attenuation of TLR4-MyD88 signaling pathway. Taken together, we propose that leucine pre-supplementation decreases LPS-induced immune damage in rohu by enhancing the expression of IL-10 and by regulating the TLR4-MyD88 signaling pathways. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Protective effects of leucine against lipopolysaccharide-induced inflammatory response in Labeo rohita fingerlings | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.fsi.2016.03.148 | - |
dc.citation.journaltitle | Fish and Shellfish Immunology | - |
dc.identifier.wosid | 000375165200025 | - |
dc.identifier.scopusid | 2-s2.0-84962215409 | - |
dc.citation.endpage | 247 | - |
dc.citation.startpage | 239 | - |
dc.citation.volume | 52 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Se Chang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | 4 SIGNALING PATHWAY | - |
dc.subject.keywordPlus | INDIAN MAJOR CARP | - |
dc.subject.keywordPlus | ACUTE LUNG INJURY | - |
dc.subject.keywordPlus | DIETARY LEUCINE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PROINFLAMMATORY CYTOKINES | - |
dc.subject.keywordPlus | AEROMONAS-HYDROPHILA | - |
dc.subject.keywordPlus | RELATIVE EXPRESSION | - |
dc.subject.keywordPlus | GROWTH-PERFORMANCE | - |
dc.subject.keywordAuthor | Leucine | - |
dc.subject.keywordAuthor | Lipopolysaccharide | - |
dc.subject.keywordAuthor | Hepatocyte | - |
dc.subject.keywordAuthor | Labeo rohita | - |
dc.subject.keywordAuthor | IL-10 | - |
dc.subject.keywordAuthor | TLR4 signaling | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.