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Impact of heart failure on the behavior of human neonatal stem cells in vitro
DC Field | Value | Language |
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dc.contributor.author | Klose, Kristin | - |
dc.contributor.author | Roy, Rajika | - |
dc.contributor.author | Brodarac, Andreja | - |
dc.contributor.author | Kurtz, Andreas | - |
dc.contributor.author | Ode, Andrea | - |
dc.contributor.author | Kang, Kyung-Sun | - |
dc.contributor.author | Bieback, Karen | - |
dc.contributor.author | Choi, Yeong-Hoon | - |
dc.contributor.author | Stamm, Christof | - |
dc.date.accessioned | 2023-05-10T01:25:25Z | - |
dc.date.available | 2023-05-10T01:25:25Z | - |
dc.date.created | 2021-05-07 | - |
dc.date.issued | 2013-09 | - |
dc.identifier.citation | Journal of Translational Medicine, Vol.11, p. 236 | - |
dc.identifier.issn | 1479-5876 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192316 | - |
dc.description.abstract | Background: Clinical cardiac cell therapy using autologous somatic stem cells is restricted by age and disease-associated impairment of stem cell function. Juvenile cells possibly represent a more potent alternative, but the impact of patient-related variables on such cell products is unknown. We therefore evaluated the behavior of neonatal cord blood mesenchymal stem cells (CB-MSC) in the presence of serum from patients with advanced heart failure (HF). Methods: Human serum was obtained from patients with severe HF (n = 21) and from healthy volunteers (n = 12). To confirm the systemic quality of HF in the sera, TNF-alpha and IL-6 were quantified. CB-MSC from healthy neonates were cultivated for up to 14 days in medium supplemented with 10% protein-normalized human HF or control serum or fetal calf serum (FCS). Results: All HF sera contained increased cytokine concentrations (IL-6, TNF-alpha). When exposed to HF serum, CB-MSC maintained basic MSC properties as confirmed by immunophenotyping and differentiation assays, but clonogenic cells were reduced in number and gave rise to substantially smaller colonies in the CFU-F assay. Cell cycle analysis pointed towards G1 arrest. CB-MSC metabolic activity and proliferation were significantly impaired for up to 3 days as measured by MTS turnover, BrdU incorporation and DAPI + nuclei counting. On day 5, however, CB-MSC growth kinetics approached control serum levels, though protein expression of cell cycle inhibitors (p21, p27), and apoptosis marker Caspase 3 remained elevated. Signal transduction included the stress and cytokine-induced JNK and ERK1/2 MAP kinase pathways. Conclusions: Heart failure temporarily inhibits clonality and proliferation of "healthy" juvenile MSC in vitro. Further studies should address the in vivo and clinical relevance of this finding. | - |
dc.language | 영어 | - |
dc.publisher | BioMed Central | - |
dc.title | Impact of heart failure on the behavior of human neonatal stem cells in vitro | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/1479-5876-11-236 | - |
dc.citation.journaltitle | Journal of Translational Medicine | - |
dc.identifier.wosid | 000325229000002 | - |
dc.identifier.scopusid | 2-s2.0-84884638957 | - |
dc.citation.startpage | 236 | - |
dc.citation.volume | 11 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kurtz, Andreas | - |
dc.contributor.affiliatedAuthor | Kang, Kyung-Sun | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CORD BLOOD | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | CAPACITY | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordAuthor | Heart failure | - |
dc.subject.keywordAuthor | Human | - |
dc.subject.keywordAuthor | Mesenchymal stromal cells | - |
dc.subject.keywordAuthor | Cord blood | - |
dc.subject.keywordAuthor | Proliferation | - |
dc.subject.keywordAuthor | Serum | - |
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