Sensitivity between Two Diagnostic Methods for Detection of Hepatopancreatic Parvovirus (HPV) Infection in Shrimp Penaeus monodon

Abstract

Sensitivity of diagnostic methods for detection HPV infection at earlier stages of shrimp is very important for the prevention of widespread and losses in shrimp hatcheries. The earliest postlarval stage detected with HPV infection was 3 days old (PL₃ The presence of hypertrophied nucleus in the hepatopancreas tubule epithelial cell diagnostic of HPV intranuclear inclusion body is indicative of HPV infection. Histological method (H&E) was significantly (P ＜ 0.01) sensitive than impression smear in the diagnosis of HPV infection in Penaeus monodon postlarvae (PL₃ to PL₂?) reared at two stocking densities (25 and 50 PL/liter). This study showed low diagnostic suitability of Giemsa impression smear and advantage of histological method when HPV infection prevalence is low. There was no significant increase in the detection rate of HPV infection in postlarval stages at two rearing densities.